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甘蔗组培苗继代培养中内源激素与绿苗生根率关系研究
引用本文:肖关丽,杨清辉,李富生,杨生超.甘蔗组培苗继代培养中内源激素与绿苗生根率关系研究[J].云南农业大学学报,2001,16(4):271-273.
作者姓名:肖关丽  杨清辉  李富生  杨生超
作者单位:肖关丽(云南农业大学农学与生物技术学院,云南昆明,650201)       杨清辉(云南农业大学农学与生物技术学院,云南昆明,650201)       李富生(云南农业大学农学与生物技术学院,云南昆明,650201)       杨生超(云南农业大学农学与生物技术学院,云南昆明,650201)
基金项目:云南省"九五"攻关资助项目
摘    要: 对甘蔗继代培养绿苗中的内源激素进行测定、分析,同时进行生根诱导。结果表明:甘蔗组培苗在继代过程中,IAA,CTK,GA1+3有积累效应,其中IAA的积累较慢,CTK,GA1+3积累在第9代后较快,同时第9代后生根率也明显下降,在第10代出现不能诱导生根的矮化丛生苗。绿苗中IAA,CTK,GA1+3的含量与生根率相关性显著。

关 键 词:甘蔗  组培苗  内源激素  绿苗生根率
文章编号:1004-390X(2001)04-0271-03
收稿时间:2001-7-10

Study on the Ralationship between EndogenousHormones and the Rooting Rate of Plantlet ofSugarcane in the Course of Subculture
XIAO Guan li,YANG Qing hui,LI Fu sheng,YANG Sheng chao.Study on the Ralationship between EndogenousHormones and the Rooting Rate of Plantlet ofSugarcane in the Course of Subculture[J].Journal of Yunnan Agricultural University,2001,16(4):271-273.
Authors:XIAO Guan li  YANG Qing hui  LI Fu sheng  YANG Sheng chao
Institution:(College of Agronomy and Biotechnology,Y A U, Kunming, 650201,China)
Abstract:Studies on the endogenous hormones and rooting induction in plantlet of sugarcane subculture were reported in this paper. It was showed that IAA, CTK and GA 1 3 of sugarcane plantlet had an accumulative effect in subculture, the IAA accumulated slower than CTK and GA 1 3 , moreover, the CTK and GA 1 3 accumulated quickly in the 9 th generation of subculture. But the rooting rate reduced in the 9 th generation of subculture. The shortened and clustered buds can't be induced to generate root in the 10 th subculture. The content of IAA, CTK and GA 1 3 in plantlet is significantly correlative with rooting rate.
Keywords:sugarcane  plantlet of tissue culture  endogenous hormones  rooting rate of plantlet
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