切花红掌炭疽病和叶霉病的病原鉴定

红掌炭疽病和叶霉病是危害切花红掌生产的两种重要病害。本研究通过传统形态学结合分子生物学方法对切花红掌生产中两种主要病害进行鉴定,并确定病原菌的分类地位。从感病植株上通过常规组织分离法获得病原菌株,对其进行形态学观察和致病性测定,通过ITS序列分析和比对,对其进行分子鉴定。结果表明:红掌炭疽病的致病菌为Colletotrichum gloeosporioides,与GenBank中查到的登录号为KC172072.1的ITS序列同源性最高,同源性大于99%;红掌叶霉病系复合侵染,其致病菌为Cladosporium cladosporioides和Alternaria alternata,分别与GenBank中查到的登录号为KC880082.1和JX406531.1的ITS序列同源性最高,同源性分别为96%和97%。分子生物学鉴定与形态学鉴定结果一致。

Pathogen Identification of Anthracnose and Leaf Mildew from Anthurium andraeanum as Cut Flower

Anthracnose and leaf mildew are major diseases which harm the production of cut flower An-thurium andraeanum.The pathogen of the two diseases was identified through traditional morphological method combined with molecular biological method .From the infected plants , the pathogenic strains were obtained through the conventional organization separation , and the morphological observation and pathogenicity identifi-cation were conducted .The molecular identification was also conducted by ITS sequence analysis and compari-son.The results suggested that Colletotrichum gloeosporioides was the pathogen of Anthurium andraeanum an-thracnose , which had the highest homology （ more than 99%） in ITS sequence with KC 172072 .1 found from GenBank.The pathogenic bacteria of Anthurium andraeanum leaf mildew were Cladosporium cladosporioides and Alternaria alternata, which had the highest homology with KC 880082 .1 and JX406531 .1 found from Gen-Bank respectively;the homology reached to 96%and 97%respectively .The molecular identification indica-ted the same results with that of morphological identification .