| 酵母CFD1基因缺失菌株构建及其对复制寿命的影响 |
| |
| 引用本文： | 袁源,;赵炜,;崔红晶,;刘新光. 酵母CFD1基因缺失菌株构建及其对复制寿命的影响[J]. 湛江医学院学报, 2014, 0(3): 280-281 |
| |
| 作者姓名： | 袁源, 赵炜, 崔红晶, 刘新光 |
| |
| 作者单位： | [1]广东医学院衰老研究所,广东东莞523808; [2]广东省医学分子诊断重点实验室,广东东莞523808; [3]广东医学院生物化学与分子生物学研究所,广东湛江524023 |
| |
| 基金项目： | 国家自然科学基金（No.31101051）; 广东医学院建博科技创新团队项目（No.STIF201102）; 广东医学院青年基金项目（No.XQ1212） |
| |
| 摘 要： | 目的测定CFD1基因缺失对酿酒酵母复制寿命的影响。方法运用基因同源重组技术构建单基因缺失酵母菌株,利用光学显微镜检测酵母细胞的复制寿命。结果与野生型酵母菌株（29.29±8.71代对比,CFD1基因缺失酵母菌株复制寿命（27.27±8.01）代有所变短,但差异无统计学意义。结论 CFD1基因缺失不影响酿酒酵母的复制寿命。）
|
| 关 键 词： | 酿酒酵母 CFD1 复制寿命 |
Construction of CFD1 deletion yeast strain and its effect on replicative life span |
| |
| Affiliation: | YUAN Yuan, ZHAO Wei, CUI Hong-jing, LIU Xin-guang (1. Institute of Aging Research, Guangdong Medical College, Dongguan 523808, China) |
| |
| Abstract: | Objective To detect the effect of CFD1 deletion yeast strain on the replicative life span. Methods The CFD1 deletion yeast strain was constructed using gene homologous recombination, and the replicative life span was determined by light microscopy. Results The replicative life span showed no significant difference between CFD1 deletion and wide type yeast strains（27.27 vs 29.29, P〉0.05）. Conclusion The CFD1 deletion has no impact on the replicative life span of Saccharomyces cerevisiae. |
| |
| Keywords: | Saccharomyces cerevisiae CFD1 replicative life span |
| 本文献已被 维普 等数据库收录！ |
|
