苗药大果木姜子总黄酮与糖类成分含量测定 |
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引用本文: | 刘刚,刘育辰,康雪佳,于福来,庞玉新,李霞,贾金燕. 苗药大果木姜子总黄酮与糖类成分含量测定[J]. 安徽农业科学, 2017, 45(3). DOI: 10.3969/j.issn.0517-6611.2017.03.048 |
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作者姓名: | 刘刚 刘育辰 康雪佳 于福来 庞玉新 李霞 贾金燕 |
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作者单位: | 贵阳中医学院,贵州贵阳550002;国家苗药工程技术研究中心,贵州贵阳550025;贵阳中医学院,贵州贵阳,550002;中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室,海南儋州571737;海南省艾纳香工程技术研究中心,海南儋州571737;贵州宏宇药业有限公司,贵州贵阳,550018 |
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基金项目: | 贵州重大课题专项计划项目 |
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摘 要: | [目的]建立测定大果木姜子中总黄酮、总糖、还原糖、多糖含量的方法。[方法]以芦丁和葡萄糖为对照品,采用紫外分光光度计测定大果木姜子中总黄酮、总糖、还原糖、多糖的含量并进行方法学考察。[结果]大果木姜子中总黄酮含量为(8.02±0.19)mg/g,总糖含量为(35.26±0.96)%,还原糖含量为(4.78±0.95)%,多糖含量为(30.53±0.69)%。[结论]该方法快速、简便、准确,可以用于大果木姜子中总黄酮、总糖、还原糖、多糖的含量测定。
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关 键 词: | 大果木姜子 总黄酮 总糖 还原糖 多糖 紫外分光光度法 |
Content Determination of Total Flavonoids and Sugar Components in Cinnamomum migao |
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Abstract: | [Objective] To establish the method for determination of total flavonoids, total sugar, reducing sugar, and polysaccharide in Cin-namomum migao.[ Method] Using rutin and glucose as control products, the contents of total flavonoids, total sugar, reducing sugar and pol-ysaccharide in C.migao were detected by UV spectrophotometer, and experiments were conducted to investigate whether the method is feasi-ble.[Result] The contents of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao were (8.02 ±0.19) mg/g, (35.26 ±0.96)%, (4.78 ±0.95)%, (30.53 ±0.69)%, respectively.[Conclusion] The method is rapid, simple, accurate, which is a-ble to accomplish the content determination of total flavonoids, total sugar, reducing sugar, polysaccharide in C.migao. |
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Keywords: | Cinnamomum migao Total flavonoids Total sugar Reducing sugar Polysaccharide UV |
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