中国部分古银杏资源遗传多样性的AFLP分析及核心种质的构建

利用AFLP技术,对来源于全国20个省(区)的180份银杏古树种质进行遗传多样性分析,在此基础上,利用逐步聚类的方法构建核心种质。结果表明,8对AFLP引物组合共扩增出1 646条谱带,多态条带百分率(PPB)为100%;平均观测等位基因数(Na)、平均有效等位基因数(Ne)、平均Nei’s基因多样度(H)和平均Shannon’s信息指数(I)分别为2.0000、1.2575、0.1662和0.2746;各种质间的遗传相似系数在0.6904~0.9115之间,平均值为0.7919。建立的银杏古树核心种质保留了原始种质的35%的样品。t检验结果表明,所构建的核心种质遗传多样性指数与原始种质差异不显著,能够最大程度的代表原始种质资源。

AFLP Analysis of Genetic Diversity and a Construction of the Core Collection of Partial Ancient Ginkgo Trees in China

Ginkgo biloba L.，endemic to China，is one of the oldest remained tree species. Studies were initiated to analyze the genetic diversity of 180 ancient ginkgo trees from 20 provinces in China based on amplified fragment length polymorphic（AFLP）marker，and a preliminary construction of the core core collection was established by using stepwise UPGMA clustering sampling approach. Eight AFLP primers selected were used to generate 1 646 discernible DNA bands，100% were polymorphic. High genetic diversity was revealed（number of different alleles，Na = 2.0000；number of effective alleles，Ne = 1.2575；Nei’s gene diversity，H = 0.1662；Shannon’s information index，I = 0.2746）. The molecular genetic similarity coefficients rang from 0.6904 to 0.9115，with an average of 0.7919. The core collection of ancient ginkgo trees with 63 individuals account for 35% of original collection. The results of t-testshowed that no significant different was found in genetic diversity indexes between the core collection and original collection. These results demonstrated that the core collection could stand for original collection excellently.