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萝卜自交不亲和基因的利用
引用本文:Nikornpun M,M.Putivoranat,钟利. 萝卜自交不亲和基因的利用[J]. 西南农业学报, 2004, 17(1): 78-83
作者姓名:Nikornpun M  M.Putivoranat  钟利
作者单位:1. 泰国清迈大学农学院,泰国,清迈,50200
2. 云南省农业科学院,云南,昆明,650221
基金项目:加拿大国际发展研究中心资助项目 
摘    要:利用荧光显微镜技术和种子结实分析方法对7个中国型萝卜(Raphanussativus L. var.longipinnatus Bailey)自交系进行研究其自交不亲和水平.试验结果表明多数自交系为自交不亲和.采取完全杂交组合方法进行筛选.从品种比较和结实情况看,杂一代27-1-3×18-1,27-1-3×18-2, 27-1-3×18-3和27-1-3×18-6 以及部分77-2×18-11 和77-4×18-7有较好的园艺性状,如整齐度、叶色深绿无毛、肉质根呈圆筒状、根皮光滑、根质紧实、根重360~400g,这些性状符合市场要求.前期试验结果表明,这些杂一代的根产量都明显高于普通品种,但还有待在农户的地里做进一步的试验.

关 键 词:自交不亲和性 荧光显微镜技术 种子结实分析 萝卜

Uses of self-incompatibility genes in Chinese radish
Nikornpun M,M.Putivoranat. Uses of self-incompatibility genes in Chinese radish[J]. Southwest China Journal of Agricultural Sciences, 2004, 17(1): 78-83
Authors:Nikornpun M  M.Putivoranat
Abstract:Seven inbred lines of Chinese radish (Raphanus sativus L. var.longipinnatus Bailey) were investigated for their self-incompatibility levels using fluorescent microscope technique and seed setting analysis. Results showed that most of the inbred lines were self-incompatible. They were crossed in all combinations to select for the best hybrid variety. From a varietal trial and an ability to set seeds, it was found that hybrids 27-1-3×18-1, 27-1-3×18-2, 27-1-3×18-3 and 27-1-3×18-6 and some of hybrids 77-2×18-11 and 77-4×18-7 had good horticultural characteristics such as uniformity, dark green leaf without hair, cylindrical shape, smooth skin, firm texture of root and root weight of about 360-400 g. These characteristics are market-acceptable. Root yield of these hybrids was shown in previous experiments that it was a lot higher than market varieties. The hybrid varieties should be further tested in farmers field.
Keywords:self-incompatibility  fluorescent microscope techniques  seed setting analysis
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