| 玉米基因组DNA的提取及SSR分析 |
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| 引用本文： | 王芳, 王化俊, 王汉宁. 玉米基因组DNA的提取及SSR分析[J]. 玉米科学, 2006, 14(2): 030-032 |
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| 作者姓名： | 王芳 王化俊 王汉宁 |
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| 作者单位： | 甘肃农业大学农学院,兰州,730070 |
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| 基金项目： | 国家高技术研究发展计划“863”计划项目(2004AA207140) |
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| 摘 要： | 采用改良的CTAB法,以玉米的黄化苗叶片为材料提取玉米基因组DNA,提取的DNA经0.8%的琼脂糖凝胶电泳检测。结果表明:此法具有快速、高效、DNA质量好和纯度高等优点。对所构建的选育高直链淀粉玉米群体的双亲进行了SSR-PCR检测,得到了清晰的多态性SSR标记,为玉米分子标记辅助育种打下了基础。
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| 关 键 词： | 玉米 DNA提取 SSR |
| 文章编号： | 1005-0906（2006）02-0030-03 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Extraction of Maize Genomic DNA and SSR Analysis |
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| WANG Fang,WANG Hua-jun,WANG Han-ning. Extraction of Maize Genomic DNA and SSR Analysis[J]. Journal of Maize Sciences, 2006, 14(2): 030-032 |
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| Authors: | WANG Fang WANG Hua-jun WANG Han-ning |
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| Affiliation: | Department of Agronomy, Gansu Agriculture University, Lanzhou 730070, China |
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| Abstract: | By using improved method of CTAB,genomic DNA of maize yellow seedling was extracted,then detected by 0.8% gelose gelatin electrophoresis in this paper.In order to detect the availability of DNA extraction approaches,the high amylose-extender maize parents were detected by SSR-PCR,too.The results showed that the improved method of CTAB had displayed many fine characteristics such as high efficiency,perfect quality and high purity,and ideal amplification bands were received.So the improved method of CTAB and SSR-PCR provided a foundation for the molecular marker-assisted maize breeding. |
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| Keywords: | Maize Genomic DNA extraction SSR |
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