| Lbx1与Lbx2基因在猪骨骼肌中的甲基化状态简 |
| |
| 引用本文： | 晁哲,孙瑞萍,魏立民,刘圈炜,郑心力,刘海隆,黄丽丽,邓昌彦,王峰. Lbx1与Lbx2基因在猪骨骼肌中的甲基化状态简[J]. 中国畜牧兽医, 2014, 41(1): 167-170 |
| |
| 作者姓名： | 晁哲 孙瑞萍 魏立民 刘圈炜 郑心力 刘海隆 黄丽丽 邓昌彦 王峰 |
| |
| 作者单位： | （1.海南省农业科学院畜牧兽医研究所，海南海口 571100；2.海南省热带动物繁育与疫病研究重点实验室，海南海口 571100；3.华中农业大学，湖北武汉 430070） |
| |
| 基金项目： | 海南省科学事业费项目（琼财预［2013］131号）；海南省自然科学基金项目（313099）；国家科技支撑计划（2012BAI39B04）。 |
| |
| 摘 要： | 为了探究猪Lbx1和Lbx2基因的甲基化模式，本研究采用亚硫酸盐测序技术，在猪背最长肌中分析Lbx1和Lbx2基因启动子和外显子1的甲基化状态。结果发现，Lbx1基因的甲基化差异区在外显子1处的CpG岛内；Lbx2基因的甲基化差异区在CpG岛外的启动子区。Lbx1基因CpG岛内的高密度甲基化可能参与下调该基因在背最长肌中的表达量。
|
| 关 键 词： | 猪 骨骼肌 甲基化 Lbx1基因 Lbx2基因 |
Characterization of the Methylation Status of Lbx1 and Lbx2 Genes in Porcine Skeletal Muscle |
| |
| CHAO Zhe,SUN Rui-ping,WEI Li-min,LIU Quan-wei,ZHENG Xin-li,LIU Hai-long,HUANG Li-li,DENG Chang-yan,WANG Feng. Characterization of the Methylation Status of Lbx1 and Lbx2 Genes in Porcine Skeletal Muscle[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(1): 167-170 |
| |
| Authors: | CHAO Zhe SUN Rui-ping WEI Li-min LIU Quan-wei ZHENG Xin-li LIU Hai-long HUANG Li-li DENG Chang-yan WANG Feng |
| |
| Affiliation: | (1. Institute of Animal Science and Veterinary Medicine, Hainan Academy of Agricultural Sciences, Haikou 571100, China；2. Hainan Key Laboratory of Tropical Animal Reproduction & Breeding and Epidemil Disease Research, Haikou 571100, China；3. Huazhong Agricultural University, Wuhan 430070, China) |
| |
| Abstract: | The study is to determine the DNA methylation status of porcine Lbx1 and Lbx2 genes. Bisulfite sequencing（BS）technique was used to analyze the specific methylation on promoter and exon 1 regions of Lbx1 and Lbx2 genes in longissimus dorsi muscle.The results showed that the differentially methylated region was found in a CpG island of Lbx1 gene exon 1, the differentially methylated region was outside of the CpG island of Lbx2 gene promoter region. These results suggested that the high DNA methylation in CpG island might be involved in the down regulation of Lbx1 gene in longissimus dorsi muscle. |
| |
| Keywords: | pig skeletal muscle methylation Lbx1 gene Lbx2 gene |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
|
点击此处可从《中国畜牧兽医》下载全文 |
|
