SlBES1调节番茄叶片形态的机制研究

为探究植物激素油菜素甾醇(BR)信号转导关键组分BES1对番茄(Solanum lycopersicum)叶片形态的调控作用,本研究构建了荧光标记微管的mBES1过表达BR超敏番茄植株,即mSlBES1-OXx SlTub6-eGFP杂交材料(B×T).形态学和组织细胞学观察发现,mBES1过表达改变了叶片细胞的形态并...

The Regulatory Mechanism of SlBES1 on Tomato Leaf Morphogenesis

To explore tomato leaf shape regulation of BES1,a key component in brassinosteroids(BRs)signaling pathway,the experiment constructed the hybrid plant,of which mBES1 gene was overexpressed and the microtubules were labeled with the enhanced green fluorescent protein(eGFP)in the mBES1-OX background(mSlBES1-OX×SlTub6-eGFP,B×T).Morphological and cytological observation showed that BES1 overexpression resulted in curly leaf and leaf cells with altered shape.Western Blot assay confirmed that BES1 overexpression reduces the expression of tubulin in tomato leaves.In addition,microtubules were more sensitive to the microtubule-disrupting regent oryzalin in B×T than in SlTub6-eGFP.In conclusion,BES1 as a key component in BR signaling pathway affects the morphology of tomato cells and leaves by regulating the protein expression of tubulin and the stability of microtubules.These results provide important insight into how BR regulates the growth and development of tomato by mediating microtubules.