手掌参多糖对60Co-γ射线辐射小鼠造血和抗氧化功能损伤的治疗作用研究

为研究手掌参多糖对电离辐射损伤小鼠造血和抗氧化功能的治疗作用,本试验选用48只健康雌性昆明小白鼠,随机分为8组,分别为空白组,辐射组,手掌参多糖低、中、高给药组(150、300、600 mg·kg^-1 组)和辐射低、中、高给药组;辐射组和辐射给药组分别用⁶⁰Co-γ射线进行辐射,辐射剂量5.0 Gy(剂量率0.8 Gy·min^-1)。分别在照射后 30 min内灌胃相应剂量的手掌参多糖和生理盐水(空白组、辐射组),连续5 d。末次灌胃后48 h内测定所有小鼠外周血细胞计数、脾脏指数、肝脏超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量、骨髓DNA含量、骨髓嗜多染红细胞微核 (MN) 数目及脾脏T淋巴细胞亚群。结果表明,与空白组相比,给药组小鼠抗氧化能力和造血系统得到不同程度的改善,辐射组外周血细胞计数、脾脏指数、骨髓DNA含量、肝脏抗氧化能力及T淋巴细胞亚群极显著降低,MDA含量与MN数目显著升高; 与辐射组相比,辐射给药组小鼠脾脏指数和T-AOC、SOD活性极显著升高,DNA含量、外周血细胞计数也有所升高,CD3⁺、CD4⁺、CD8⁺ T淋巴细胞比率升高,MDA含量、MN数目显著降低。上述试验结果表明,手掌参多糖对⁶⁰Co-γ射线照射造成的小鼠造血、抗氧化功能损伤具有治疗作用。本研究为手掌参多糖对辐射损伤的治疗提供了技术参考。

Therapeutic Effect of Tibetan Medicine Gymnadenia conopsea Polysaccharide on Hematopoietic and Anti-Oxidative Damage in Mice Irradiated With 60Co-γ Rays

In order to study the therapeutic effect of gymnadenia conopsea polysaccharide on the hematopoietic and antioxidant function of mice injured by ionizing radiation, 48 healthy female Kunming mice were selected and randomly divided into 8 groups, i. e., the blank group, the irradiation group, the gymnadenia conopsea polysaccharide administration group with the low, medium and high doses (150, 300, 600 mg·kg^-1) and the gymnadenia conopsea irradiation administration group with the low, medium and high doses. The blank group and the administration group were not irradiated. The irradiation group and the irradiation administration group were irradiated with ⁶⁰Co-γ rays at a radiation dose of 5.0 Gy (dose rate 0.8 Gy·min^-1). The gymnadenia conopsea polysaccharide and physiological saline (blank group and irradiated group) were given intragastrically within 30 min after irradiation for 5 consecutive days. Within 48 hours after the last gavage, the peripheral blood, organ index, liver superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, bone marrow DNA content, dismutase (SOD) activity, bone marrow polychromatic erythrocyte micronucleus (MN) number and splenic T lymphocyte subsets of all mice were measured within 48 hours after the last intragastric administration. Compared with the blank group, the anti-oxidation ability and hematopoietic system of the administration group were improved to varied degrees. The peripheral blood cell count, spleen index, bone marrow DNA content, liver antioxidant capacity and T lymphocyte subsets of the irradiated group were significantly reduced, while the MDA content and the MN number, the spleen index, T-AOC and SOD activity in the irradiation administration group were significantly increased; DNA content, and peripheral blood cell count also increased. The CD3⁺, CD4⁺, CD8⁺ T lymph cell ratio increased while the MDA content and the number of MN decreased significantly (P<0.05). The experimental results show that the gymnadenia conopsea polysaccharide has a therapeutic effect on the damage to the hematopoietic and anti-oxidant functions of mice caused by ⁶⁰Co-γ ray irradiation. This study provides a technical reference for the treatment of radiation damage caused using palmar ginseng polysaccharides.