蒲公英不同部位抑菌作用及代谢物差异的UPLC-MS/MS分析

为了研究蒲公英不同部位抑菌作用和代谢物成分的差异,本物采用打孔法和超高效液相色谱串联质谱(UPLC-MS/MS)方法,研究了蒲公英根、茎叶和花的水提取物和醇提取物对5种致病菌的抑菌作用,并对不同部位的代谢物成分进行了定性和定量分析。结果表明,蒲公英不同部位初提取物对变形杆菌、嗜根考克氏菌和枯草芽孢杆菌均有抑菌作用,而对大肠杆菌和铜绿假单胞菌均无抑菌作用;醇提法的抑菌效果优于水提法,花提取物的抑菌活性最强,根最弱。蒲公英的不同部位共鉴定出449种代谢物,根、茎叶和花中相对含量最高的物质分别为反油酸(脂类)、γ-亚麻酸(脂类)和木犀草素(黄酮类);且根、茎叶和花中相对含量排名前20的物质中黄酮类分别有0种、3种和8种,表明黄酮类物质可能是蒲公英中的主要抑菌活性物质。正交偏最小二乘法-判别分析(OPLS-DA)结果显示,木犀草素-7-O-葡萄糖苷在花中上调幅度最大,橙皮素C-丙二酰己糖苷为根中特有物质。本研究结果为利用蒲公英不同部位研制和开发新型药物提供了理论依据。

Analysis of Bacteriostatic Effect and Metabolite Differences by UPLC-MS/MS From Different Parts of Dandelion

To compare the effects aqueous and alcoholic extracts of dandelion root, stem and leaf, and flower, on bacteria activity and metabolite composition across five types bacteria. The antibacterial activity was determined using the drilling method, whereas the metabolite composition was analyzed by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS). The results showed that the initial extracts of different parts of dandelion showed antibacterial effects against Proteus hauser, Kocuria rhizophila, and Bacillus subtilis, but not on Escherichia coli and Pseudomonas aeruginosa. The antibacterial activity of the alcoholic extract was higher compared to that of the aqueous extract, and flower extracts had the strongest antibacterial activity, while root extracts had the weakest activity. A total of 449 metabolites were identified in the dandelion root, stem and leaf, and flower, among which elaidic acid (lipids), γ-linolenic acid (lipids), and luteolin (flavonoids), had the highest concentration, in that order. Zero, two, and eight flavonoids were among the top 20 most abundant substances in roots, stems and leaves, and flowers, respectively, indicating that flavonoids may be the main active substances in dandelion responsible for its antibiotic activity. The results of orthogonal partial least squares discriminant analysis (OPLS-DA) showed that luteolin-7-O-glucoside was the abundant substance in flowers whereas hesperetin C-malonyl hexoside was only in the roots. The results of this study provide a theoretical basis for the development and exploitation of different parts of T.mongolicum Hand.Mazz for drug development.