| 基于PSY2基因单碱基突变的木薯薯肉颜色分子标记开发与利用 |
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| 引用本文： | 朴朴森,尚小红,许丰收,廖明馨,王明,严华兵,陈新,王文泉. 基于PSY2基因单碱基突变的木薯薯肉颜色分子标记开发与利用[J]. 核农学报, 2022, 36(1): 34-41. DOI: 10.11869/j.issn.100-8551.2022.01.0034 |
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| 作者姓名： | 朴朴森 尚小红 许丰收 廖明馨 王明 严华兵 陈新 王文泉 |
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| 作者单位： | 1中国热带农业科学院热带作物生物技术研究所,海南 海口 5711012广西农业科学院经济作物研究所,广西 南宁 5300073海南大学热带作物学院,海南 海口 5702284中国热带农业科学院热带作物品种资源研究所,海南 海口 5711015缅甸教育部研究与创新部生物技术研究院,缅甸 曼德勒 05151 |
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| 基金项目： | 国家重点研发计划(2019YFD100098、2018YFD1000501)； |
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| 摘 要： | 木薯块根颜色是消费者关注的重要性状之一,薯肉颜色主要受八氢番茄红素酶PSY2基因控制.为了快速判断木薯块根肉质颜色,本研究基于编码八氢番茄红素合成酶基因的突变位点PSY2-572,开发出1个单核苷酸扩增多态性(SNAP)标记,可快速检测木薯块根肉质颜色.利用该PSY2-SNAP标记鉴定出的新选048自交群体和62个育种...
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| 关 键 词： | 木薯 分子标记辅助选择 八氢番茄红素合成酶 块根颜色 单碱基突变 |
| 收稿时间： | 2020-10-21 |
Development and Utilization of Cassava Root Color Marker Basing on a Single Nucleotide Mutation in PSY2 Gene |
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| PHYU Phyu Thin,SHANG Xiaohong,XU Fengshou,LIAO Mingxin,WANG Ming,YAN Huabing,CHEN Xin,WANG Wenquan. Development and Utilization of Cassava Root Color Marker Basing on a Single Nucleotide Mutation in PSY2 Gene[J]. Acta Agriculturae Nucleatae Sinica, 2022, 36(1): 34-41. DOI: 10.11869/j.issn.100-8551.2022.01.0034 |
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| Authors: | PHYU Phyu Thin SHANG Xiaohong XU Fengshou LIAO Mingxin WANG Ming YAN Huabing CHEN Xin WANG Wenquan |
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| Affiliation: | (Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101;Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007;Tropical Crops College,Hainan University,Haikou,Hainan 570228;Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101;Biotechnology Research Department,Department of Research and Innovation,Ministry of Education,Mandalay 05151,Myanmar) |
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| Abstract: | The cassava root color is one of the important traits to attract consumers.Root color is mainly determined by the Phytone Synthase 2 in cassava.In order to determine the color of flesh cassava root efficiently,a single nucleotide amplified polymorphic(SNAP)marker was developed based on the mutation of PSY2-572 in PSY2,which can be used to detect the flesh color of cassava root quickly.The genotypes of Xinxuan 048 inbred population and 62 breeding intermediate materials identified by the PSY2-SNAP were consistent with the phenotype we observed.If the PSY-F1/PSY-R primer pair could amplify a 388 bp PCR product,the cassava root would be white flesh,if the PSY-F2/PSY-R primer pair could amplify a 388 bp PCR product,the cassava root would be dark yellow,if both of PSY-F1/PSY-R and PSY-F2/PSY-R primer pairs could amplify 388 bp PCR product,the cassava would be yellow flesh.In addition,the genotypes of 45 edible lines in the natural cassava nursery were identified by PSY2-SNAP,and some new yellow-color cassava germplasms were found.In summary,the PSY2-SNAP developed in this study is a valuable color molecular marker of cassava root in breeding,which can lay a theoretical foundation for molecular marker assisted selection of cassava. |
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| Keywords: | cassava molecular marker assisted selection octahydro lycopene synthase root color single base mutation |
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