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黄秋葵中黄酮类化合物提取方法优化及总黄酮抑菌效果研究
引用本文:陈晓琪,李瑶晨,李璐瑶,杨静,饶帅琦,祝彪,臧运祥,周根.黄秋葵中黄酮类化合物提取方法优化及总黄酮抑菌效果研究[J].核农学报,2022,36(3):669-679.
作者姓名:陈晓琪  李瑶晨  李璐瑶  杨静  饶帅琦  祝彪  臧运祥  周根
作者单位:浙江农林大学农业与食品科学学院,浙江 杭州 311300
基金项目:浙江省重点研发计划项目(2017C02021);
摘    要:为高效提取黄秋葵中黄酮类化合物,本试验以黄秋葵中的4种主要黄酮类化合物和黄秋葵总黄酮含量为响应值,通过单因素试验和Box-Behnken响应面试验进行提取工艺优化,并采用牛津杯法考察黄秋葵总黄酮对6种常见致病菌的抑菌活性.结果表明,在提取温度37℃、提取时间20 min、乙醇浓度70%、料液比1:25 g·mL-1的条...

关 键 词:黄秋葵  黄酮  响应面法  高效液相色谱法  抑菌效果
收稿时间:2020-12-15

Optimization by Response Surface Methodoloy of Flavonoids Extraction and the Antibacterial Activities of the Total Flavonoids From Okra(Abelmoschus esculentus L.)
CHEN Xiaoqi,LI Yaochen,LI Luyao,YANG Jing,RAO Shuaiqi,ZHU Biao,ZANG Yunxiang,ZHOU Gen.Optimization by Response Surface Methodoloy of Flavonoids Extraction and the Antibacterial Activities of the Total Flavonoids From Okra(Abelmoschus esculentus L.)[J].Acta Agriculturae Nucleatae Sinica,2022,36(3):669-679.
Authors:CHEN Xiaoqi  LI Yaochen  LI Luyao  YANG Jing  RAO Shuaiqi  ZHU Biao  ZANG Yunxiang  ZHOU Gen
Institution:College of Agriculture and Food Science, Zhejiang Agricultural and Forestry University, Hangzhou, Zhejiang 311300
Abstract:To extract flavonoids more efficiently,the contents of four individual main flavonoids and the total flavonoids of okra(Abelmoschus esculentus L.)were obtained using single-factor test and response surface methodology with Box-Behnken design.The antibacterial activities of the total flavonoids in okra against six common pathogens were also investigated by the Oxford cup method.The content of the total flavonoids of okra was 4576.1μg·g-1,including quercetin-3-gentiobioside(2403.7μg·g-1),quercetin-3-O-β-D-xylosyl-(1→2)-β-D-glucoside](516.5μg·g-1),isoquercetin(1170.7μg·g-1)and quercetin-3-O-(6-O-malonyl)-β-D-glucoside(485.2μg·g-1)under the optimal extraction conditions,that the extraction temperature 37°C,extraction time 20 min,ethanol concentration 70%,and solid-to-solvent ratio 1∶25 g·mL-1.The values of the flavonoids were close to the predicted value,indicating the feasibility of the extraction process.The extracted flavonoids exhibited good antibacterial activities against Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Canidia albicans,Aspergillusniger niger and Rhizopus nigricans.The minimum inhibitory concentration(MIC)was 5.0,3.8,5.0,3.8,7.5 and 7.5 mg·mL-1,respectively.The results provided a theoretical basis for the further development and utilization of okra flavonoids.
Keywords:okra  flavonoid  response surface  high performance liquid chromatography  bacteriostatic effect
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