甘肃甘南传统发酵牦牛乳中细菌菌群的多样性研究

为了研究甘肃甘南传统发酵牦牛乳中细菌菌群的多样性,本试验以甘肃甘南地区传统发酵牦牛乳为研究对象,基于Illumina MiSeq高通量测序技术,对13份发酵牦牛乳的16S rRNA中V3-V4区进行测序,分析甘肃甘南地区传统发酵牦牛乳中细菌菌群的多样性.结果表明,最终获得720 221条高质量16S rRNA基因序列,...

The Bacterial Community Diversity of Traditional Fermented Yak Milk in Gannan of Gansu

To investigate the bacterial diversity of the traditional fermented yak milk in the Gannan area of Gansu province,the V3-V4 regions of 16 S rRNA from 13 fermented yak milks were sequenced using Illumina MiSeq high-throughput sequencing technology to analyze the bacterial flora diversity of traditional fermented yak milks in Gannan,Gansu Province.The results showed that 720221 high-quality 16 S rRNA gene sequences were generated and four different bacterial phyla and four different bacterial genera were identified.Firmicutes was the dominant phylum,Lactobacillus was the high abundance genus,and yak yogurt from different sources showed different abundance at the phylum level.PICRUSt analysis based on 16 S rRNA sequencing data of each sample revealed that the basic predicted function accounted for 11.76%of all genes.This study can build a basis for the protection and utilization of microbial resources in the traditional fermented yak milk in Gannan,Gansu.