黄瓜耐盐种质资源的筛选与评价

为了明确黄瓜种质对氯化钠的不同耐性,解析黄瓜耐盐的分子机理,本研究以耐盐种质MC2065、盐敏感种质白黄瓜和21份其他黄瓜种质为材料,设置5个NaCl浓度,50、100、150、200、250 mmol·L^-1分别开展适宜NaCl浓度筛选,以及适宜浓度处理后耐盐指数、叶绿素、保护酶等9个生理生化指标的测定与分析。结果表明,100和150 mmol·L^-1可以作为耐盐鉴定的适宜浓度。NaCl浓度为100 mmol·L^-1时,白黄瓜盐害指数为35.94%,出现明显盐害胁迫,而MC2065盐害指数为4.69%,未表现出明显盐害症状;NaCl浓度为150 mmol·L^-1时,两个材料均表现明显盐害症状,白黄瓜出现半数以上叶片枯萎,而MC2065出现半数以下叶片枯萎。聚类分析发现,21份材料分为3个类群,第一类群包括ZQ3、N26-5-1、寿水1、DRTJY-2、莱西、翠玉、20S077-1,属于耐盐种质;第二类群包括DJ04、DY-1、XY1、YY9123、M2XT、D1503、X805,属于中耐盐种质;第三类群包括F6-3-1、SJ11-1、XB23、20S091-1、HLT-921h、AZ-1、DRT345,属于盐敏感种质。主成分分析发现,黄瓜种质综合得分趋势与聚类分析、黄瓜幼苗存活率高低趋势基本一致。本研究结果为黄瓜耐盐机理的研究提供了参考,也为黄瓜耐盐品种的育种工作奠定了基础。

Screening and Evaluation of Cucumber Salt-Tolerant Germplasm

In order to clarify the different tolerance of cucumber germplasm to NaCl, the salt-tolerant material MC2065 and salt-sensitive material White cucumber were used to verify the appropriate concentration of 50, 100, 150, 200, and 250 mM NaCl. And nine physiological and biochemical indexes, including salt tolerance index, chlorophyll, and protective enzymes under NaCl treatment were used to evaluate the salt-tolerances of 21 germplasms. The results showed that 100 and 150 mM could be the appropriate concentration.The salt injury index of White cucumber was 35.94% in 100 mM NaCl, showing obvious damage to leaves, while that of MC2065 was 4.69% and showed no obvious damage. Under 150 mM, both showed obvious damage, more than half of leaves were withered for White cucumber, but less than half for MC2065. With cluster analysis, 21 germplasms were divided into 3 groups. The first group categorized as salt-tolerant germplasm, including ZQ3, N26-5-1, Shoushui 1, DRTJY-2, Laixi, Cuiyu, 20S077-1. The second group, including DJ04, DY-1, XY1, YY9123, M2XT, D1503, X805, was medium salt-tolerant germplasm. And the third group was salt-sensitive germplasm, which included F6-3-1, SJ11-1, XB23, 20S091-1, HLT-921h, AZ-1, DRT345. The trend of comprehensive scores of cucumber germplasm by the compoment analysis was basically consistent with that obtained by cluster analysis and seedlings survival rate. The study will provide references for investigating cucumber salt-tolerant mechanism and lay a foundation for the breeding of cucumber salt-tolerant varieties.