E-cadherin在牦牛不同繁殖周期输卵管不同部位中的表达与定位

为研究上皮钙粘蛋白(E-cadherin)在不同繁殖周期牦牛输卵管不同部位中的表达差异,本研究采集卵泡期、黄体期和妊娠期无临床病理表现的母牦牛(3～6岁)输卵管伞部、壶腹部和峡部的样品,将不同繁殖周期输卵管分别按照伞部、壶腹部和峡部分为9组,采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法(western-bl...

Expression and Localization of E-cadherin in Different Parts of the Oviduct at Different Reproduction Cycles of Yak

The objective of this study was carried out to study the expression differences of E-cadherin in different parts of the oviduct at different reproduction cycles of yak,and to provide some important theoretical foundations for understanding the effect of E-cadherin on it′s reproductive performance.The samples of the tubal umbrella,ampulla,and isthmus of female yaks(3~6 years old)with no clinical pathological manifestations during follicular,luteal and pregnant phases were collected,the different reproduction cycles of yak are divided into 9 groups according to the umbrella,ampulla and isthmus.Real-time PCR(qRT-PCR)and western-blotting were used to detect the expression of E-cadherin gene and protein.The expression of E-cadherin protein was localized by immunohistochemistry.The results showed that during follicular phase,the expression of E-cadherin gene and protein in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus.During luteal phase,the expression of E-cadherin gene and protein in the isthmus of the oviduct was significantly higher than that in the umbrella and ampulla.During pregnant phase,the expression of E-cadherin gene in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus,and the expression of E-cadherin protein in the ampulla of the oviduct was significantly higher than that in the umbrella In the oviduct umbrella,the expression of E-cadherin gene in the luteal phase was significantly higher than that in the follicular phase and the pregnant phase,and the expression of E-cadherin protein in the follicular phase was significantly higher than that in the luteal phase and the pregnant phase.In the oviduct ampulla,the expression of E-cadherin gene and protein in the pregnant phase was significantly higher than that in the follicular phase and luteal phase.In the oviduct isthmus,the expression of E-cadherin gene and protein in the luteal phase was significantly higher than that in the follicular phase and pregnant phase.The results of immunohistochemistry showed that E-cadherin protein was positively expressed in ciliated cells,secretory cells,basal cells,muscular layers and serous glands of the oviduct umbrella,ampulla and isthmus.The results suggested that E-cadherin in different parts of the oviduct at reproduction cycles of the yak was located,and the expression of E-cadherin gene and protein was significantly different,which may be involved in a series of reproductive processes such as the growth and maturation of oocytes,sperm storage and capacitation,fertilization and early embryonic development of yak,and provided basic information for further clarifying of the biological effect of E-cadherin on yak′s reproductive performance.