朱红橘果实成熟过程中类胡萝卜素的积累及相关基因的表达分析

为探究朱橘类果实成熟过程中类胡萝卜素的积累及相关基因的表达模式,本研究以朱红橘为材料,使用手持色差仪测定果实成熟过程中果皮和果肉的色差值,利用液相色谱串联质谱(LC-MS/MS)检测类胡萝卜素组分的含量变化,并利用实时荧光定量PCR(qRT-PCR)分析类胡萝卜素代谢相关基因的表达.结果表明,朱红橘成熟过程中,果皮和果...

Carotenoids Accumulation and Relative Genes Expression During the Maturation of Zhuhong Mandarin

Carotenoids is an important compound widely distributed in nature and citrus fruit is a rich source of carotenoids.This study explored the accumulation of the carotenoids and the expression of related genes during the ripening of zhuhong mandarin based on following methods:color index value of peel and pulp was evaluated using a handheld chromaticmeter;the carotenoids composition and content were detected by LC-MS/MS and qRT-PCR was used to analyze the expression of carotenoid synthesis-related genes.The results showed that the Color Index increased during the ripening process,and the Color Index in peel was much higher than pulp at fully mature.The content of carotenoid showed a similar trend as Clor Index,but some carotenoid ingredients in the peel and pulp was different.We found thatβ-citraurin and Apocarotenal were detected in peel,but not in pulp.The analysis of qRT-PCR found that the expressions of carotenoid related genes were basically up-regulated except PDS,LCYE,and ZEP genes.The expression of PSY,ZDS and HYD genes were significantly related to the content of carotenoid.This study provides a basis for the development and utilization of functional components in citrus.