非洲栽培稻基因渗入系粒形和剑叶形态性状的QTL定位

非洲栽培稻作为重要的水稻种质资源,其基因渗入系可以为普通栽培稻的遗传背景提供新的有利基因,如果能将这些优良基因引入普通栽培稻中,可为水稻分子设计育种提供新的基因资源。本研究以非洲栽培稻基因渗入系YIL60与轮回亲本中9B(Z9B)杂交衍生的包含188个株系的F₂和F_2:3群体为材料,对粒形性状包括粒长、粒宽、籽粒长宽比、千粒重,剑叶形态性状包括剑叶长、剑叶宽进行数量性状点位(QTL)检测。结果共检测到16个QTL,包括2个粒长QTL、3个粒宽QTL、2个籽粒长宽比QTL、2个千粒重QTL、1个剑叶长QTL、6个剑叶宽QTL,分布于第1、第6、第7、第10和第11号染色体上,贡献率为2.25%~25.64%;有4个多效性QTL区间,有4个QTL qGW7-1、qFLL10、qFLW10、qTGW7在F₂和F_2:3群体中被重复检测到,其中在第7号染色体RM3859-RM3394区间检测到同时控制粒长、粒宽、籽粒长宽比和千粒重的QTL,贡献率最高达17.10%,是一个来源于非洲栽培稻的新粒形QTL。本研究为进一步开展粒形、剑叶形态性状基因的精细定位、克隆和分子标记辅助育种工作奠定了一定的理论基础。

QTL Mapping of Grain Shape and Flag Leaf Morphology Traits of an African Cultivated Rice(Oryza glaberrima)Introgression Line

African cultivated rice(Oryza glaberrima)is an important rice germplasm resource.Its introgression line can provide new beneficial genes to the genetic background of ordinary cultivated rice.If these excellent genes can be introduced into Asian cultivated rice,it will provide valuable gene resources for molecular design breeding of high-yield and high-quality rice.In this study,the F₂and F_2:3populations of 188 lines derived from the cross of the introgression line YIL60 and the recurrent parent Zhong9 B(Z9 B)were used as materials.The grain shape traits including grain length(GL),grain width(GW),the ratio of grain length to width(LWR),thousand-grain weight(TGW),flag leaf morphological traits including flag leaf length(FLL)and flag leaf width(FLW)were tested and the QTLs were mapped.A total of 16 QTLs were detected,including 2 QTLs for GL,3 QTLs for GW,2 QTLs for LWR,2 QTLs for TGW,1 QTL for FLL,and 6 QTLs for FLW,these QTLs were distributed on chromosomes 1,6,7,10 and 11,which could explain 2.25%~25.64%of the phenotypic variation.There were four pleiotropic QTL intervals,and four QTLs qGW7-1,qFLL10,qFLW10,qTGW7 were detected repeatedly in both F;and F;populations.Among them,the locus between RM3589 and RM3394 on chromosome 7 simultaneously controlling GL,GW,LWR and TGW was identified,and explained up to 17.10%of the phenotypic variation,which is a novel grain shape QTL from Africa cultivated rice.These results will lay a certain theoretical foundation for further fine mapping,cloning of genes and molecular marker-assisted breeding for the grain shape and flag leaf traits.