生草栽培对南丰蜜橘园土壤微生物群落结构与功能特征的影响

为探究橘园生草对土壤微生物群落结构与功能特征的影响,本研究以南丰蜜橘(Citrus reticulita Blanco cv.Kinokuni)为研究对象,设置行间播种白三叶草(Trifolium repens)、黑麦草(Lolium multiflorum)、自然生草和清耕4个处理,采用荧光定量PCR技术和Illum...

Effects of Grass Cultivation on Soil Microbial Community Structure and Functional Characteristics in Nanfeng Tangerine Orchard

In order to explore the effects of grass cultivation on soil microbial community structure and functional characteristics,the study took Nanfeng tangerine as the research object and was conducted with four treatments:sod culture with white clover(Trifolium repens),sod culture with ryegrass(Lolium multiflorum),sod culture with natural grass and clear tillage as control.Fluorescence quantitative PCR and Illumina HiSeq high-throughput sequencing were employed to examine the structure and functional characteristics of soil microbial community under different treatments.The results showed that grass cultivation was beneficial to increase the supply of soil organic matter and nitrogen,phosphorus,potassium,and improved the soil fertility.The ACE index of bacterial community increased significantly in sod culture with ryegrass and white clover.The Chao1 index and Simpson index of fungal community increased significantly in sod culture with ryegrass.The above results indicated that artificial grass cultivation could increase the richness and diversity of microbial community,compared with natural grass and clear tillage.A total of 24 bacterial phyla and 9 fungal phyla were obtained from four soil samples under different grass treatments,of which the dominant bacterial phyla were Proteobacteria(30.94%~55.06%),Acidobacteria(19.07%~33.14%),Chloroflexi(7.54%~13.54%)and Actinobacteria(6.81%~13.41%)and the dominant fungal phyla were Ascomycota(44.94%~87.00%),Basidiomycota(5.12%~12.99%)and Mortierellomycota(0.65%~13.46%).The principal coordinates analysis showed that differences between groups of bacterial and fungal communities were much greater than those within groups.Correlation analysis showed that soil organic matter,available phosphorus,total potassium,alkali-hydrolysable nitrogen content and pH had greater impact on bacterial community structure,while organic matter,available phosphorus,total phosphorus,available potassium and total nitrogen content had greater impact on fungal community structure.In conclusion,sod culture with ryegrass and white clover not only improved the nutrient supply of soil nitrogen,phosphorus and potassium,but also increased the richness and diversity of microbial community,optimized the community structure of soil bacterial and fungal,promoted the sustainable development of Nanfeng tangerine industry.