| 欧洲黑莓的组织培养研究 |
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| 引用本文： | 薛陈心,赵锦,刘孟军.欧洲黑莓的组织培养研究[J].河北农业大学学报,2007,30(2):34-37. |
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| 作者姓名： | 薛陈心 赵锦 刘孟军 |
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| 作者单位： | 1. 河北农业大学,中国枣研究中心,河北,保定,071001
2. 河北农业大学,生命科学学院,河北,保定,071001 |
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| 基金项目： | 中波政府间科技合作项目 |
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| 摘 要： | 以欧洲黑莓优良品种冬福瑞(Thornfree)冬季休眠枝为外植体,建立了其快繁体系,即初代培养基为MS+6-BA 1.0 mg/L;增殖培养基为MS+6-BA 0.5 mg/L+IBA 0.1 mg/L;生根培养基为MS+IBA 0.5-1.0 mg/L;在此体系中增殖系数可达6.5,生根率100%。利用此体系获得的生根组培苗进行移栽,移栽成活率可达到100%,为黑莓工厂化育苗和应用提供理论基础。
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| 关 键 词： | 欧洲黑莓 冬福瑞 组织培养 激素 移栽 |
| 文章编号： | 1000-1573(2007)02-0034-04 |
| 修稿时间： | 2006年9月5日 |
Tissue culture of European blackberry (Rubus fruticosus cv. Thornfree) |
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| XUE Chen-xin,ZHAO Jin,LIU Meng-jun.Tissue culture of European blackberry (Rubus fruticosus cv. Thornfree)[J].Journal of Agricultural University of Hebei,2007,30(2):34-37. |
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| Authors: | XUE Chen-xin ZHAO Jin LIU Meng-jun |
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| Abstract: | The buds from water cultured dormant branches of Eurpoean blackberry(Rubus fruticosus cv.Thornfree) were employed as explants in this experiment.The optimum media for initialing,proliferating,and rooting culture were established,i.e.,MS 6-BA 1.0 mg/L,MS 6-BA 0.5 mg/L IBA 0.1 mg/L and MS IBA 0.5-1.0 mg/L respectively.In this system,the highest propagation coefficient was 6.5 and the rooting rate was 100%.The rooted plantlets were transplanted to the glasshouse and then field,and the survived plantlets rate of transplant was 100%,which was a theoretical basis of the industrial breeding and application for blackberry. |
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| Keywords: | European blackberry(Rubus fruticosus cv Thornfree) thornfree tissue culture hormone transplant |
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