生长素相关基因在茶树腋芽冬季休眠不同生长阶段的表达分析

利用实时定量PCR技术研究了从本实验室构建的茶树休眠芽与萌动芽抑制消减杂交文库中分离出来的与生长素相关的基因片段在茶树腋芽冬季休眠不同阶段的表达模式.研究结果表明:(1)与生长素响应因子同源的6条EST片段中,有5条的表达量休眠期高于萌发期,另外1条则相反.(2)与生长素原初反应基因同源的3条EST片段的表达模式存在差异,其中与SAURs同源的EST在休眠阶段上调表达,休眠解除以后下调表达.而与GH3和Aux/IAAs同源的2条EST在萌发阶段有一个表达高峰,而在休眠期和萌发以后,表达量都下降.(3)与生长素结合类蛋白同源的茶树CsGLP1表达量在萌发期高于休眠期.(4)与生长素内向运输载体基

Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant(Camellia sinensis)

The expression patterns of some auxin-related genes,including auxin response factors(ARFs),primary auxin responsive genes,auxin-regulated genes,auxin influx carrier gene and auxin-binding protein(ABP) gene,were studied in different stages of tea plant axillary bud dormancy and its release.The results showed that(1) the expression levels of five in six ARF homologous EST fragments were up-regulated in the deep dormancy and down-regulated after sprouted,the level of the last one showed the opposite expression levels.(2) There were significant differences among the expression patterns of 3 ESTs which are homologous with primary auxin responsive genes.EST which is homologous with SAURs was up-regulated in the dormancy stage but down-regulated after dormancy release.On the contrary,the 2 ESTs which are homologous with GH3 and Aux/IAAs were up-regulated in sprouting stage while down-regulated in dormant stages and after sprouting stages.(3) The CsGLP1 gene which was homogenous with ABPs has a lower expression level in the dormancy stage but maintain rising trend after germination.(4) The EST which is homogenous with auxin influx carrier gene showed the highest expression levels during the deep dormant stage but maintain lower levels in other stages.(5) Two auxin-regulated ESTs showed different expression patterns.The expression levels of auxin-induced EST were higher in the dormant stages than those in the sprouting stages,while the levels of the other auxin-repressed EST were higher in the sprouting stage than that in the dormant stages.These results indicated that auxin promote the dormancy of tea axillary buds in winter.