豆渣纤维改性多糖的抗氧化活性研究 |
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引用本文: | 姚磊,王振宇,赵海田,程翠林,富校轶,孙树坤. 豆渣纤维改性多糖的抗氧化活性研究[J]. 东北农业大学学报, 2012, 0(11): 16-19 |
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作者姓名: | 姚磊 王振宇 赵海田 程翠林 富校轶 孙树坤 |
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作者单位: | 哈尔滨工业大学食品科学与工程学院;国家大豆工程技术研究中心东北农业大学;东北林业大学林学院 |
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基金项目: | 国家自然科学基金项目(31170510,31000831) |
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摘 要: | 利用酶法降解豆渣纤维,得到不同时间段的豆渣纤维改性多糖,对其清除ABTS自由基、亚铁离子螯合能力进行评价。结果表明,降解时间2、4、6、8、10、12 h大豆多糖组分对ABTS自由基有一定的清除效果,降解12 h组对ABTS自由基清除效果最好,EC50值为(34.62±1.21)mg·mL-1;对亚铁离子有较好的螯合作用,并呈现剂量依赖性,2 h组大豆多糖组分对亚铁离子螯合能力最强,5 mg·mL-1浓度时,螯合率达到62.92%±2.31%。
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关 键 词: | 豆渣 改性多糖 抗氧化 ABTS自由基 亚铁离子螯合 |
Antioxidant activities of polysaccharides from soybean meal |
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Affiliation: | YAO Lei1, 2, WANG Zhenyu1,3,ZHAO Haitian1,CHENG Cuilin1,FU Xiaoyi2,SUN Shunkun2(1.School of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090,China;2.National Research Center of Soybean Engineering and Technology,Northeast Agriculture University,Harbin 150030,China;3.School of Forestry,Northeast Forestry University,Harbin 150040,China) |
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Abstract: | Soybean meal modified polysaccharides(SMP)was made by enzymatic hydrolyzed of soybean meal fiber.Ferrous ion chelating activity and ABTS radical scavenging activity of SMP were evaluated.The results showed that,SMP obtained after different hydrolyzing times(2,4,6,8,10,12 h) had effective ABTS radical scavenging activity and ferrous ions chelating activity,and the effects showed significant dose dependence.The EC50 value of 12 h group was(34.62±1.21) mg·mL-1,and the rate of chelation 5 mg·mL-1 concentration was 62.92%±2.31%. |
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Keywords: | soybean meal modified polysaccharides antioxidant activity ABTS radical ferrous ion chelating |
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