| 固相萃取结合高效液相制备茶树没食子酸衍生物 |
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| 引用本文： | 卢忠尉,蒋晓岚,刘亚军,刘莉,高丽萍,夏涛.固相萃取结合高效液相制备茶树没食子酸衍生物[J].茶叶科学,2012,32(6):494-499. |
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| 作者姓名： | 卢忠尉 蒋晓岚 刘亚军 刘莉 高丽萍 夏涛 |
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| 作者单位： | 1. 安徽农业大学生命科学学院,安徽 合肥 230036; 2. 安徽农业大学 教育部茶叶生物化学与生物技术重点实验室,安徽 合肥 230036 |
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| 基金项目： | 国家自然科学基金(No.30972401、No.31170647和No.31170282);安徽省自然科学基金(11040606M73) |
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| 摘 要： | 没食子酸衍生物是茶树酚酸中的主要成分,是茶汤中重要的鲜味增强剂,具有抗过敏、抗抑郁和增强认知能力等作用。利用固相萃取（solid-phase extraction,SPE）结合制备高效液相色谱（preparative high performance liquid chromatography,PHPLC）,从茶树鲜叶中分离制备出高纯度的3-O-没食子酰奎尼酸和β-D-没食子酰葡萄糖,并利用LC/TOF-ESI-MS、1H-NMR和13C-NMR对它们的结构进行了鉴定。该方法操作简单、纯化效果好。
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| 关 键 词： | 没食子酸衍生物 固相萃取 制备高效液相色谱 β-D-没食子酰葡萄糖 3-O-没食子酰奎尼酸 |
| 收稿时间： | 2012-01-06 |
Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant |
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| LU Zhong-wei
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JIANG Xiao-lan
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LIU Ya-jun
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LIU Li
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GAO Li-ping
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XIA Tao.Preparation of Gallic Acid Derivatives by Solid-phase Extraction Cartridge Coupled with Preparative High Performance Liquid Chromatography from Tea Plant[J].Journal of Tea Science,2012,32(6):494-499. |
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| Authors: | LU Zhong-wei JIANG Xiao-lan LIU Ya-jun LIU Li GAO Li-ping XIA Tao |
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| Institution: | 1. School of Biology Science, Anhui Agricultural University, Hefei 230036, China; 2. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, China |
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| Abstract: | Gallic acid derivatives are the chief compounds of phenolic acid in tea.They are important umami-enhancing compounds in tea beverage,and possess the effects of inhibiting IgE producton,enhancing cognition-anting depressant.A reversed-phase PHPLC coupled with SPE was developed to purify and to prepare high purity 3-O-galloyquinic acid and β-D-glucogalline from fresh tea leaves.The obtained fractions were successfully identified by LC/TOF-ESI-MS,1H-NMR and 13C-NMR.This method is simple,effective for the preparation of gallic acid derivatives. |
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| Keywords: | gallic acid derivatives SPE PHPLC β-D-glucogalline 3-O-galloyquinic acid |
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