基于LC-MS技术高通量筛选产Cardiogenol C的芽胞杆菌

Cardiogenol C是目前治疗心脏疾病的一种重要药物,本研究采用LC-MS的技术高通量从51株芽胞杆菌中筛选产Cardiogenol C的菌株。利用MassHunter软件对分子特征进行提取,根据保留时间及精确分子量等信息在Metlin代谢数据库中进行快速的比对,高通量的对代谢产物进行鉴定。筛选鉴定到1株解淀粉芽胞杆菌FJAT-17934 Bacillus amyloliquefaciens产Cardiogenol C,其相对含量为1.02%,匹配度达到了92.08%,保留时间为3.651 7min。这为微生物源的Cardiogenol C的研发奠定理论基础。

High Throughput Screening of Cardiogenol C-producing Bacillus-like Strains Based on LC-MS

Cardiogenol C is an important medicine for treating cardiovascular diseases.To search for a biological source for the substance, a high throughput method base on LC-MS was carried out to screen the potential Bacillus-like strains.The molecular information on the target metabolites was collected using the MassHunter software.Accordingly, substances with their retention times and molecular weights that matched those on the Metlin database were identified.Bacillus amyloliquefaciens FJAT-17934 was, thus, isolated to show a high production level of cardiogenol C in its culture medium with a relative content of 1.02%.The matching score with the database for the cardiogenol C collected was 92.08%, and the retention time, 3.6517 min.