| 大黄鱼鱼卵磷脂酰胆碱的分离纯化 |
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| 引用本文： | 程新伟,梁鹏,孙鹤,赵卉双,金红,程文健,陈丽娇. 大黄鱼鱼卵磷脂酰胆碱的分离纯化[J]. 福建农林大学学报(自然科学版), 2017, 46(2). DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2017.02.017 |
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| 作者姓名： | 程新伟 梁鹏 孙鹤 赵卉双 金红 程文健 陈丽娇 |
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| 作者单位： | 1. 福建农林大学食品科学学院,福建 福州,350002;2. 福建农林大学食品科学学院,福建 福州 350002;福建农林大学海洋研究中心,福建 福州 350002 |
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| 基金项目： | 福建省自然科学基金资助项目，福建省教育厅中青年教师A类项目，福建农林大学高水平大学建设项目 |
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| 摘 要： | 以氯仿/甲醇法提取的大黄鱼鱼卵粗磷脂为原料,研究柱层析固定相种类、粒度,洗脱剂流速、配比及样品上载量等因素对分离纯化的鱼卵磷脂酰胆碱(Roe-PC)纯度和回收率的影响,以确定最佳的工艺参数.结果表明,在固定相为200~300目硅胶,流动相为氯仿/甲醇(3∶2),流速为2 m L·min~(-1),上载量为0.035 g·g~(-1)(样品/硅胶)的条件下,分离纯化的大黄鱼Roe-PC的纯度可达96.34%,回收率为85.18%.
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| 关 键 词： | 大黄鱼鱼卵 磷脂酰胆碱 分离纯化 |
Isolation and purification of phosphatidylcholine from large yellow croaker roe |
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| CHENG Xinwei,LIANG Peng,SUN He,ZHAO Huishuang,JIN Hong,CHENG Wenjian,CHEN Lijiao. Isolation and purification of phosphatidylcholine from large yellow croaker roe[J]. Journal of Fujian Agricultural and Forestry University, 2017, 46(2). DOI: 10.13323/j.cnki.j.fafu(nat.sci.).2017.02.017 |
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| Authors: | CHENG Xinwei LIANG Peng SUN He ZHAO Huishuang JIN Hong CHENG Wenjian CHEN Lijiao |
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| Abstract: | Phosphatidylcholine ( PC) was purified from phospholipids of large yellow croaker ( Pseudosciaena crocea) roe by chloro-form/methanol system. Parameters including types of stationary phase, granularity, eluent rate and composition, loading quantity were investigated to optimize its yield and purity. The results showed that the optimum procedure was to co-eluate sample from silica gel 200-300 using chloroform/methanol (3 : 2) at the rate of 2 mLmin-1, with loading amount being 0.035 gg-1(sample/silica gel) . Under this condition, the purity of PC can reach 96.34%, with recovery rate being 85.18%. |
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| Keywords: | large yellow croaker roe phosphatidylcholine purification |
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