IBA与ABT组合对喜树插条内源激素变化的影响

采用酶联免疫法(ELISA),研究了不同浓度IBA与ABT组合对喜树嫩枝扦插生根效应及插条内源激素变化的影响。结果表明:与对照相比,IBA和ABT组合可显著提高叶及基部茎段内源激素IAA、ZR含量和IAA/ABA、ZR/ABA比值;降低GA3、ABA含量。进而提高喜树插穗成活率。各处理中,ABT 1 000+IBA1 000mg·L-1浸泡20s效果最好,处理后第40d插穗叶、茎内源IAA含量分别比对照增加57.5%、77.4%;叶内GA3含量第30d显著低于对照24.8%;茎段GA3含量第40d显著低于对照35.0%;叶、茎内ABA含量第30d时分别比对照降低19.2%、32.2%。叶、茎段内IAA/ABA第20、30d出现最高值,分别比对照提高114.0%、150.0%。叶、茎段内ZR/ABA变化与IAA/ABA变化类似,分别第20、30d达到峰值,较对照分别提高151.4%、39.9%。IAA/ABA、ZR/ABA可作为衡量喜树插穗成活质量的2个指标。

Effect of IBA and ABT on Endogenous Hormones in Rooted Cuttings of Common Camptotheca Fruit (Camptotheca acuminata Decne)

Cuttings of young branches from common camptotheca fruit(Camptotheca acuminata Decne)plants were treated by ABT and IBA to study their effect on the endogenous hormones in the cuttings.Responding changes on abscisic acid (ABA), indole-acetic acid (IAA), zeatin riboside (ZR), and gibberellins (GA3) in the leaves and stems of the plantlets by the treatmentswere determined using the enzyme-linked immunosorbent assay (ELISA).The results showed that soaking the cuttings in ABT and IBA solutions in varied concentrationsimproved the rooting over control.The contents of IAA and ZR, as well as the ratios of IAA/ABA and ZR/ABA, in the leaves and stems increased significantly after the pre-treatment.But, those of GA3, ABA, reduced.Among all treatments, by dipping the cuttings in a 1,000 mg ABT/L+1,000 mgIBA/L solution for 20s,the highest survival rate was obtained.IAA in the leaves and the stems peaked on the 40thday, i.e., an increase by 57.5% and 77.4%, respectively, as compared to that of control.At the same time, GA3 in the leaves decreased by 24.8% over control.Onthe 30th day, GA3 in the stems declined 35.0%, ABA in the leaves and the stems decreased by 19.2% and 32.2%, respectively, over control.The maximal IAA/ABA ratio appeared on the 20thday in the leaves and 30thday in the stems, representing the increases of 114.0% and 150.0%, respectively, in comparison to control;and, similarly, the peak ZR/ABA in the leaves and the stems.It appeared that the ratios ofIAA/ABA and ZR/ABA could be used as an index for the survival quality of camptotheca cuttings.