Cryogenic preservation of spermatozoa from Prochilodus scrofa and Salminus maxillosus |
| |
| Authors: | Ana Maria Coser Hugo Godinho Dirceu Ribeiro |
| |
| Affiliation: | 1. Department of Morphology, Federal University of Minas Gerais, Belo Horizonte Brazil;2. Furnas Centrais Elétricas, Minas Gerais Brazil |
| |
| Abstract: | This paper reports an initial trial to cryopreserve semen from two freshwater South American fishes, the curimbatá (Prochilodus scrofa) and the dourado (Salminus maxillosus). Motility and duration of motility were observed in curimbatá and dourado fresh sperm. Semen mixed with extender (0.8% NaCl) was frozen using vials (1 ml) with subsequent storage in liquid nitrogen. Samples were thawed in 1% NaHCO3 or in 0.8% NaCl solutions. Post-thawing motility and duration of motility were verified. A simple extender consisting of 0.8% NaCl plus 10% DMSO was able to initiate motility in fresh spermatozoa. The percentage of motile cells and duration of motility were similar in both thawing solutions, but lower than in fresh sperm. |
| |
| Keywords: | To whom all correspondence should be addressed. |
| 本文献已被 ScienceDirect 等数据库收录！ |
