Rearing of marine fish larvae in Japan: Katsuzo Kuronuma and Kunihiko Fukosho. IDRC,Ottawa, Ont., 1984. 109 pp., illus., ISBN 0-88936-406-0, IDRC-TS47e (also available as microfiche)

Serotonin induced spawning in six bivalve species using individual spawning techniques without any additional stimuli. Intragonadal injection of serotonin induced spawning in the bay scallop Argopecten irradians, the American oyster Crassostrea virginica, and the surf clam Spisula solidissima. Injection of serotonin into the anterior adductor muscle of the ocean quahog Arctica islandica, the ribbed mussel Geukensia demissa, and the hard clam Mercenaria mercenaria induced spawning. The dosage of 0.4 ml of 2 mM serotonin solution used in this study stimulated bivalves to spawn within 15 min.