| 湘棉18茎尖培养条件初步研究 |
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| 引用本文： | 薛远超,刘大军. 湘棉18茎尖培养条件初步研究[J]. 安徽农学通报, 2007, 14(16): 40-41. DOI: 10.3969/j.issn.1007-7731.2007.16.016 |
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| 作者姓名： | 薛远超 刘大军 |
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| 作者单位： | 西南大学农学与生物科技学院,重庆,400715;西南大学农学与生物科技学院,重庆,400715 |
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| 摘 要： | 以腺体突变型湘棉18为试验材料,先将棉花种子在无菌的蛭石中培养5-7d后,取棉花苗制备棉花苗茎尖,培养在无激素MSB培养基或加入激素的MSB培养基上,直接再生完整植株,结果表明:植株再生率主要与再生培养基中外源激素含量、活性炭浓度及茎尖分生组织的取材时间密切相关.
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| 关 键 词： | 棉花 转基因 组织培养 茎尖分生组织 植株再生 |
| 文章编号： | 1007-7731（2007）16-40-02 |
| 收稿时间： | 2007-07-31 |
| 修稿时间： | 2007-07-31 |
Initill study on Tip Tissue Cultural Condition of Xiangmian 18 Cotton |
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| XUE Yuanchao,Liu Dajun. Initill study on Tip Tissue Cultural Condition of Xiangmian 18 Cotton[J]. Auhui Agricultural Science Bulletin, 2007, 14(16): 40-41. DOI: 10.3969/j.issn.1007-7731.2007.16.016 |
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| Authors: | XUE Yuanchao Liu Dajun |
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| Affiliation: | College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China |
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| Abstract: | The present paper as tests the material take the gland mutant xiangmian 18 cotton and kapok ,and Carries on the stem point meristematic tissue raise. Transfers the gene by this material achievement the preparation material . The cotton seed were excisedfrom the seedlings of 5 -7 days and culturedon the MSB medium without/with hormone oractive charcoal. The rate of regenerated plantletattached little to the tested cotton varieties, butconnected with the ingredients of regenerated medium , such as horm one or active charcoal. |
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| Keywords: | cotton particle bom bardment tissue culture apical meristems plant regeneration |
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