氟吡菌酰胺和吡唑醚菌酯在葡萄上的残留及膳食风险评估

为明确氟吡菌酰胺和吡唑醚菌酯在葡萄上的残留风险，开展了一年十地的规范残留试验，建立了高效液相色谱法(HPLC)测定葡萄中氟吡菌酰胺和吡唑醚菌酯残留的分析方法。结果表明，在0.1～20.0 mg·L^-1范围内，氟吡菌酰胺和吡唑醚菌酯质量浓度与其峰面积均呈良好的线性关系，相关系数大于0.99。在0.05、0.50 mg·kg^-1和2.00 mg·kg^-1添加水平下，氟吡菌酰胺和吡唑醚菌酯在葡萄中的平均回收率分别为88.1%～112.3%和90.5%～101.5%，相对标准偏差(RSD)分别为3.1%～8.2%和3.5%～9.1%，检测方法定量限(LOQ)均为0.05 mg·kg^-1。采收间隔期为14 d，时葡萄中氟吡菌酰胺和吡唑醚菌酯的残留中值(STMR)分别为0.16 mg·kg^-1和0.12 mg·kg^-1，最高残留值(HR)分别为1.44 mg·kg^-1和1.48 mg·kg^-1。葡萄中氟吡菌酰胺和吡唑醚菌酯对...

Residues and dietary risk assessment of fluopyram and pyraclostrobin in grape

To determine the residue risks of fluopyram and pyraclostrobin on grapes, the supervised residue trials were conducted in 10 regions within 1 year. A highly efficient and simple method was developed for the determination of fluopyram and pyraclostrobin in grapes using high-performance liquid chromatography（HPLC）. The results demonstrated that a good linear relationship between the peak area and the concentration of fluopyram and pyraclostrobin was observed in the range of 0.1-20.0 mg·L^-1 with a correlation coefficient greater than 0.99. The average recoveries of fluopyram and pyraclostrobin in grapes at three spiked levels of 0.05, 0.50, and 2.00 mg·kg^-1 were 88.1%-112.3% and 90.5%-101.5%, with the relative standard deviations（RSDs）ranged from 3.1%-8.2% and 3.5%-9.1%, respectively. The limits of quantification（LOQs）of the two analytes in grape were all 0.05 mg·kg^-1. The supervised trial median residues（STMR）of fluopyram and pyraclostrobin in grapes were 0.16 and 0.12 mg ·kg^-1, and the highest residues（HR）were 1.44 and 1.48 mg ·kg^-1, respectively, at 14 d after the last application. According to the dietary risk assessment, the contribution rates of fluopyram and pyraclostrobin in grapes to the long-term dietary risk of the general population were 0.142% and 0.036%, the short-term dietary intake risk quotients were 7.8% and 5.7%, and the short-term dietary intake risk quotients for children aged 1-6 years were 19.6% and 14.4%, respectively. These results show that the residues of fluopyram and pyraclostrobin in grapes would not pose significant health risks to children aged 1-6 years and the general population when fluopyram and pyraclostrobin formulation are applied at the recommended dose.