A novel canine B‐cell leukaemia cell line. Establishment,characterisation and sensitivity to chemotherapeutics |
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| Authors: | A Pawlak E Ziolo J Kutkowska A Blazejczyk J Wietrzyk A Krupa W Hildebrand P Dziegiel S Dzimira B Obminska‐Mrukowicz L Strzadala A Rapak |
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| Institution: | 1. Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland;2. Laboratory of Tumor Molecular Immunobiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland;3. Laboratory of Experimental Oncology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wroclaw, Poland;4. Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland;5. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland;6. Department of Pathology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw, Poland |
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| Abstract: | We established a new B‐cell leukaemia cell line CLB70 from a dog with chronic lymphocytic leukaemia. This cell line is positive for CD20, CD45, CD79a, MHC class II, IgG, IgM; weakly positive for CD21; and negative for CD3, CD4, CD5, CD8, CD14, CD34, CD117. PCR for antigen receptor gene rearrangement (PARR) analysis revealed a biclonal immunoglobulin heavy chain (IgH) gene rearrangement and negative result for TCRγ. Western blot analysis of anti‐ and pro‐apoptotic proteins showed increased expression of Bcl‐2, Mcl‐1, NF‐kB, and Ras, and decreased expression of p53. CLB70 cells grow rapidly in vitro and are tumourigenic in nude mice. The CLB70 line is highly sensitive to doxorubicin, less sensitive to etoposide and imatinib, and resistant to piroxicam, celecoxib and dexamethasone. Our results indicate that CLB70 cells are derived from mature B‐cells and they may be a useful tool for the development of new therapeutic strategies for both dogs and humans. |
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| Keywords: | B‐CLL canine cell line canine leukaemia xenograft |
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