S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce Tenascin C synthesis under DMSO exposure in vitro |
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| Authors: | S Kau I Miller A Tichy C Gabriel |
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| Institution: | 1. Institute of Anatomy, Histology and Embryology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria;2. Institute for Medical Biochemistry, Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria;3. Platform Biostatistics, Department of Biomedical Science, Institute of Population Genetics, University of Veterinary Medicine, Vienna, Austria |
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| Abstract: | In breast cancer research S100A4‐positive tumour‐associated stromal cells are assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4/TNC positive stromal canine mammary tumour (CMT) cells. Cells grown as scaffold‐free spheroids were investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well‐known side effects on cells including TNC expression. DMSO did not affect proliferation, but TNC was significantly reduced under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was only observed after 14 days. Without DMSO, cells stably expressed TNC and S100A4 which makes them suitable to be used in experimental approaches requiring S100A4/TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not be used as solvent for experiments concerning TNC/S100A4 expression. |
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| Keywords: | canine mammary tumour DMSO S100A4 TNC tumour stroma |
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