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Tissue Culture of the Enteric Nervous System from Equine Ileum
Authors:Hudson  NPH  Pearson  GT  Mayhew  IG
Institution:(1) Departments of Preclinical Veterinary Sciences and Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, EH9 1QH, UK;(2) Department of Preclinical Veterinary Sciences, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, EH9 1QH, UK;(3) Department of Veterinary Clinical Studies, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Edinburgh, EH9 1QH, UK
Abstract:Ileal samples were harvested fresh from euthanized adult horses. The tissues were microdissected to prepare wholemount preparations for immunohistochemistry and for either explant or dissociated culture systems of the enteric nervous system. Explant culture systems were established using wholemounts of either the submucous plexus or the muscularis externa (including the myenteric plexus). Dissociated cell cultures could only be obtained from the submucous plexus. Culture systems were maintained for up to 5 days. Immunoreactivity for a neuronal marker (Pan-N) and for glial cell markers (GFAP and S100) indicated the presence of both neurons and enteric glia in the tissue culture preparations.This is the first report of equine enteric neurons being grown in tissue culture. Further refinements to the techniques will be required before this in vitro model can be used for quantitative analysis.
Keywords:culture  enteric nervous system  glia  horse  ileum  immunohistochemistry  neuron  submucous plexus
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