Quantity, motility and fertility of tench Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments |
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Authors: | Nicolas Caille Marek Rodina Martin Kocour David Gela Martin Flajšhans Otomar Linhart |
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Institution: | (1) Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, University of South Bohemia, Research Institute of Fish Culture and Hydrobiology, 38925 Vodnany, Czech Republic;(2) University of Nancy 1, INPL, INRA, Laboratory Science Animales, MAN, 34 Rue St Catherine, Nancy, F-5400, France |
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Abstract: | The spermiation of tench males was stimulated with Supergestran containing mammalian LHRHa lecireline at the following doses:
5, 10, 20 and 40 g kg−1 b.w.; then with carp pituitary suspension (CPS) at a dose of 2 mg kg−1 b.w. and with a control of saline physiological solution. The following days, meaning 24, 48 and 72 h after injection, sperm
was collected to evaluate volume and the number of sperm per male per kg body weight (B.W.) The percentage of motile sperm
and velocity of spermatozoa were measured 48 h after hormonal injection, and 72 h after hormonal injection the sperm was evaluated
for fertilization and hatching ability. All 42 males in experimental groups were diploid. Live weight did not differ significantly
among experimental groups. The strongest stimulation of spermiation was achieved with LHRHa in dosage of 20 and 40 g kg−1 b.w. and CPS compared to males of the control group and lower dosage of LHRHa. Analysis of variance showed no significant
influence of the treatment on the velocity and percentage of motile spermatozoa. The effect of different treatment on the
fertilization capacity (the number of spermatozoa per egg was equilibrated) was significant. Significantly the highest quality
of sperm collected 72 h after injection expressed by percentage of fertilization and hatching (62–65% fertilization and 61–64%
hatching rates, respectively) was found for LHRHa in dosage of 20 and 40 g kg−1 b.w. Significantly the lowest parameters of fertilization and hatching were found for the control group, on the 12% level. |
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Keywords: | Aquaculture Fish Hormone Motility Reproduction Sperm Spermatozoa Tinca tinca |
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