| LIR1基因在水稻中的组织特异性表达 |
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| 引用本文： | 岳彩黎,王贵学,黄俊丽,胡锋,秦峰. LIR1基因在水稻中的组织特异性表达[J]. 南京农业大学学报, 2012, 35(2): 146-150 |
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| 作者姓名： | 岳彩黎 王贵学 黄俊丽 胡锋 秦峰 |
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| 作者单位： | 重庆大学生物工程学院应用生物技术中心,重庆,400030 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08009-3109B);国家自然科学基金项目(30900882);重庆市国内科技合作项目(2007AC1060) |
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| 摘 要： | 水稻LIR1是LIR(light-induced rice)蛋白家族的一员,受光与生物钟的调节,在植物光反应及生物节律性调控方面有重要作用。为了研究水稻LIR1的生理功能,利用半定量RT-PCR技术对水稻‘珍汕97B’LIR1基因做了根、叶鞘、叶片及穗的组织特异性表达分析,同时构建了启动子的GUS基因融合表达载体LIR1∷GUS转化烟草,利用GUS组织化学染色检测GUS基因在烟草组织器官中的表达情况。研究结果表明:LIR1基因在水稻叶片中的表达量较高,而在叶鞘、穗与根中表达量较低;GUS染色主要集中在叶片组织及茎中,而在植株的根部不显色。
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| 关 键 词： | LIR1基因 组织特异性表达 启动子 GUS |
Tissue-specific expression of LIR1 gene in rice |
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| YUE Cai-li , WANG Gui-xue , HUANG Jun-li , HU Feng , QIN Feng. Tissue-specific expression of LIR1 gene in rice[J]. Journal of Nanjing Agricultural University, 2012, 35(2): 146-150 |
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| Authors: | YUE Cai-li WANG Gui-xue HUANG Jun-li HU Feng QIN Feng |
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| Affiliation: | (Research Center of Applied Biotechnology,College of Biological Engineering,Chongqing University,Chongqing 400030,China) |
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| Abstract: | Rice LIR1,a member of LIR(light-induced,rice)protein family,regulated by light and the biological clock,plays an important role for photosynthesis and biorhythm.In order to study the physiological functions of LIR1,the tissue-specific expression analysis of LIR1 gene in root,leaf sheath,young leaves,mature leaves and young panicle was carried out by RT-PCR,and the fusion binary expression vector LIR1∷GUS was constructed to study the expression of GUS in transgenic tobacco plants.The results demonstrated that the expression level of LIR1 gene was much higher in leaf than that in leaf sheath,young panicle and root,and GUS expression was mainly concentrated in the stem,the major veins of leaf and the mesophyll presenting uneven distribution,but not in root. |
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| Keywords: | LIR1 gene tissue-specific expression promoter GUS |
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