| 不同产区麦冬、山麦冬rDNA-ITS序列分析 |
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| 引用本文： | 黄玉吉,陈菁瑛,苏海兰,黄颖桢,万学锋.不同产区麦冬、山麦冬rDNA-ITS序列分析[J].福建农业学报,2009,24(6):508-512. |
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| 作者姓名： | 黄玉吉 陈菁瑛 苏海兰 黄颖桢 万学锋 |
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| 作者单位： | 1. 福建省农业科学院农业生物资源研究所,福建,福州,350003
2. 福建省农业科学院农业生物资源研究所,福建,福州,350003;福建农林大学园艺植物生物工程研究所,福建,福州,350002 |
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| 基金项目： | 国家科技支撑计划项目，福建省农业科学院青年科技人才创新基金项目 |
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| 摘 要： | 利用特异性引物进行PCR扩增和克隆、测定不同产区麦冬和山麦冬原植物的18S～26S r DNA-ITS区碱基序列,结果得到供试的7个不同产区麦冬和山麦冬植株样品rDNA的ITS及5.8S rDNA全序列、18S和26S rDNA部分序列,序列长度在696～711 bp.通过分析比较各ITS序列,表明不同产区麦冬和山麦冬rDNA-ITS区存在差异,可为其分子鉴别提供了依据.
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| 关 键 词： | 麦冬 山麦冬 |
rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions |
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| HUANG Yu-ji,CHEN Jing-ying,SU Hai-lan,HUANG Ying-zhen,WAN Xue-feng.rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions[J].Fujian Journal of Agricultural Sciences,2009,24(6):508-512. |
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| Authors: | HUANG Yu-ji CHEN Jing-ying SU Hai-lan HUANG Ying-zhen WAN Xue-feng |
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| Institution: | HUANG Yu-ji1,CHEN Jing-ying1,SU Hai-lan1,HUANG Ying-zhen1,WAN Xue-feng1,2(1.Institute of Agrobiological Resources,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China,2.Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fujian 350002,China) |
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| Abstract: | The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS.The complete sequences of ITS and 5.8S rDNA,as well as partial sequences of 18S rDNA and 26S rDNA,in the range of 696-711 bp for the seven samples were obtained.By comparing the sequences,it was apparent that the ITS sequences of R.ophiopogonis and R.liriopes from different regions varied.Our analytical results provided a basis for molecular identificat... |
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| Keywords: | rDNA-ITS |
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