马生长激素基因多态性与体尺指标之间的关联性分析

为研究广西德保矮马、百色马和新疆伊犁马的生长激素(GH)基因多态性与体尺指标之间的相关性,本实验利用PCR-SSCP技术分析3个品种277匹马GH基因5′侧翼区、第3外显子、第4外显子、第5外显子的遗传多态性。结果表明:只有GH基因第5外显子出现多态性,表现为AA、BB和AB 3种基因型,其他片段未发现多态性。群体遗传学分析表明,等位基因A和B的基因频率相等;Hardy-Weinberg平衡检验显示,3个品种马均处于非平衡状态;它们的多态信息含量均为中度多态。统计分析表明,GH基因第5外显子的基因型与3个品种马体尺指标之间无显著相关性。

Association of Polymorphisms of Growth Hormone Gene with Body Sizes Indexes in Horses

The genetic polymorphisms of 5′-flanking region,3rd exon,4th exon and 5th exon of GH gene in 277 horses from three breeds including Debao pony,Baise horse and Yili horse were investigated using PCR-SSCP method,to analyze the association of the polymorphism of GH gene with body size indexes in these horses.The results showed that only the 5th exon has polyrnorphism which generated three genotypes: AA,BB and AB;the rest fragments’polyrnorphisms are undetectable.Analysis of population genetics indicated that the gene frequency of allele A and B are equal among the studied breeds.Hardy-Weinberg equilibrium testing showed that 3 breeds did not reach the equilibrium.These breeds had intermediate polymorphism information content.The statistical analysis indicated the genotypes of the 5th exon of GH gene were no significant relation with the body size indexes among 3 breeds.