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| 三个花生二酰甘油酰基转移酶基因的克隆与序列分析 | |
| 引用本文： | 迟晓元,雷永,陈娜,王通,王冕,陈明娜,潘丽娟,杨珍,禹山林. 三个花生二酰甘油酰基转移酶基因的克隆与序列分析[J]. 花生学报, 2014, 0(4): 1-12 |
| 作者姓名： | 迟晓元 雷永 陈娜 王通 王冕 陈明娜 潘丽娟 杨珍 禹山林 |
| 作者单位： | 1. 农业部油料作物生物学与遗传育种重点实验室 /中国农业科学院油料作物研究所，湖北 武汉 430062; 山东省花生研究所，山东 青岛 266100 2. 农业部油料作物生物学与遗传育种重点实验室 /中国农业科学院油料作物研究所,湖北 武汉,430062 3. 山东省花生研究所,山东 青岛,266100 |
| 基金项目： | 国家花生产业体系，山东省三院联合基金，国家自然基金，农业部油料作物生物学与遗传育种重点实验室开放课题基金 |
| 摘 要： | 二酰甘油酰基转移酶在植物油脂合成途径中具有重要的作用。本研究通过构建花生种子全长cDNA文库,结合大规模EST测序和RACE克隆等方法,从花生中克隆得到了三个DGAT基因,分别命名为AhDGAT1-1、AhDGAT1-2和AhDGAT3-3。其中AhDGAT1-1全长为2020bp,ORF为1539bp,理论分子量为58.6036kD,理论等电点为8.83;AhDGAT1-2全长为2553bp,ORF为1581bp,理论分子量为60.6598kD,理论等电点为8.94;AhDGAT3-3全长为1377bp,ORF为1023bp,理论分子量为36.911kD,理论等电点为8.17。将这三个基因在GenBank上注册,注册号分别为KC736068,KC736069和KC736067。 |
| 关 键 词： | DGAT 花生 基因克隆 序列分析 |
Cloning and Sequence Analysis of Three Diacylglycerol Acyltransferase (DGAT) Genes in Peanut |
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| CHI Xiao-yuan,LEI Yong,CHEN Na,WANG Tong,WANG Mian,CHEN Ming-na,PAN Li-juan,YANG Zhen,YU Shan-lin. Cloning and Sequence Analysis of Three Diacylglycerol Acyltransferase (DGAT) Genes in Peanut[J]. Journal of Peanut Science, 2014, 0(4): 1-12 | |
| Authors: | CHI Xiao-yuan LEI Yong CHEN Na WANG Tong WANG Mian CHEN Ming-na PAN Li-juan YANG Zhen YU Shan-lin |
| Affiliation: | CHI Xiao-yuan, LEI Yong, CHEN Na, WANG Tong, WANG Mian, CHEN Ming-na, PAN Li-juan, YANG Zhen, YU Shan-lin (1. Key Lab. of Biology and Genetic Improvement of Oil Crops, Ministry of Agr. / Oil Crops Research Institute, Chinese Academy of Agr. Sci. , Wuhan, 430062, China; 2. Shandong Peanut Research Institute, Qingdao 266100, China) |
| Abstract: | Diacylglycerol acyltransferase （DGAT） is a key enzyme in biosynthesis pathway of veg-etable oil in plant.We constructed a full-length cDNA library of peanut （Arachis hypogaea L.） seed via a large number of sequences of expressed sequence tag （EST） and RACE clone, three diacylglycer-ol acyltransferase genes, designated AhDGAT 1-1, AhDGAT 1-2 and AhDGAT 3-3 were isolated from peanut.The sequence of AhDGAT 1-1 cDNA was 2020 bp, and ORF was 1539 bp.The molecular weight of AhDGAT1-1 was 58.6036 kD and its isoelectric point （pI） was 8.83.The sequence of Ah-DGAT 1-2 and AhDGAT 3-3 cDNA were 2553 bp and 1377 bp, and ORF were 1581 bp and 1 1023 bp,＆amp;nbsp;respectively.The molecular weight of AhDGAT1-2 and AhDGAT3-3 were 60.6598 kD and 36.91 1 kD, and isoelectric points （pI） were 8.94 and 8.17, respecively.The sequences of the three genes were also submitted to GenBank and their GenBank ID were KC736068, KC736069, KC736067, re-spectively. |
| Keywords: | diacylglycerol acyltransferase peanut (Arachis hypogaea L.) gene cloning se-quence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |