| 不同方法处理对椰子花粉扫描电镜观察结果的影响 |
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| 引用本文： | 李和帅,范海阔.不同方法处理对椰子花粉扫描电镜观察结果的影响[J].江西农业学报,2011,23(5):74-75,81. |
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| 作者姓名： | 李和帅 范海阔 |
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| 作者单位： | 中国热带农业科学院椰子研究所、海南省热带油料作物生物学重点实验室,海南文昌,571339 |
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| 基金项目： | 海南省自然科学基金项目，公益性行业科技项目 |
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| 摘 要： | 分别用冻干、常温干燥器干燥、36℃烘干、液氮冷冻、-80℃冷冻等5种方法对海南高种椰子花粉的电子扫描显微镜观察样品进行预处理。结果表明:以冻干法的效果最好;常温干燥器干燥与36℃烘干法制备的花粉样品在扫描电镜下观察呈现塌陷、变形状;-80℃冷冻与液氮冷冻处理的雄花外壁呈褐色,花药、花丝潮湿,电镜观察视野中的杂质、碎片较多。
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| 关 键 词： | 椰子 花粉 电子扫描显微镜 样品制备 观察 |
Effect of Different Sample Treatment Methods on Observation of Coconut Pollen by Scanning Electron Microscope |
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| LI He-shuai,FAN Hai-kuo.Effect of Different Sample Treatment Methods on Observation of Coconut Pollen by Scanning Electron Microscope[J].Acta Agriculturae Jiangxi,2011,23(5):74-75,81. |
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| Authors: | LI He-shuai FAN Hai-kuo |
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| Institution: | (Coconut Research Institute,China Academy of Tropical Agricultural Sciences;Hainan Key Laboratory of Tropical Oil Crops Biology,Wenchang 571339,China) |
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| Abstract: | Five methods(freeze-drying,silica-gel drying at room-temperature,36 ℃ drying,liquid nitrogen freezing,-80 ℃ freezing) were used to pretreat the male flowers of coconut,and then scanning electron microscope(SEM) was used to observe the morphology of the pollen.The results showed that the freeze-drying pretreatment had the best effect for the observation of coconut pollen,and the exine ornamentations of the pollens pretreated by freeze-drying were clear.While some of the pollens pretreated by 36 ℃ drying or s... |
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| Keywords: | Coconut Pollen SEM Preparation of sample Observation |
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