| C型口蹄疫病毒衣壳蛋白前体P1基因的原核表达及其生物活性的初步分析 |
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| 引用本文： | 常惠芸,丛国正,独军政,邵军军,林彤,冯金瑞,刘萍.C型口蹄疫病毒衣壳蛋白前体P1基因的原核表达及其生物活性的初步分析[J].华北农学报,2009,24(5). |
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| 作者姓名： | 常惠芸 丛国正 独军政 邵军军 林彤 冯金瑞 刘萍 |
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| 作者单位： | 1. 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃,兰州,730046
2. 中国农业科学院,兰州兽医研究所,家畜疫病病原生物学国家重点实验室,农业部畜禽病毒学重点开放实验室,国家口蹄疫参考实验室,甘肃,兰州,730046;中农威特生物科技股份有限公司,甘肃,兰州,730046 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 将口蹄疫病毒(FMDV)结构蛋白基因P1的完整cDNA序列插入原核表达性载体pET-28α(+)中,获得融合表达质粒pET-P1,转化E.coli.BL21(DE3),经IPTG诱导,SDS-PAGE结果表明,pET-P1获得融合表达, Western Blot 检测证实表达的融合蛋白具有免疫活性,表达产物主要以包涵体的形式存在,进一步采用纯化试剂盒纯化P1蛋白做为诊断抗原.
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| 关 键 词： | C型口蹄疫病毒 重组蛋白P1 表达生物活性 |
Expression of the Capsid:Precursor Polypeptide P1 of Foot-and-mouth Disease Virus Type C and Analysis of Its Biology Activity |
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| CHANG Hui-yun,CONG Guo-zheng,DU Jun-zheng,SHAO Jun-jun,LIN Tong,FENG Jin-rui,LIU Ping.Expression of the Capsid:Precursor Polypeptide P1 of Foot-and-mouth Disease Virus Type C and Analysis of Its Biology Activity[J].Acta Agriculturae Boreali-Sinica,2009,24(5). |
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| Authors: | CHANG Hui-yun CONG Guo-zheng DU Jun-zheng SHAO Jun-jun LIN Tong FENG Jin-rui LIU Ping |
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| Abstract: | The complete gene encoding the structural protein of FMDV( P1 ) was subcloned into expression vector pET-28o( + ),resulting in the fusion expression plasmid pET-Pl .After transformed into E. coli (DE3) and induced by IPTG,the results of SDS-PAGE showed that the fusion protein was expressed in high level.The molecular weight of the fusion protein was 85 kDa and the expressed products were significant at inclusion body. Western blotting was performed to confirm that the expressed fusion protein could specifically react with antiserum against FMDV. The fusion protein were further purified and vaccined antigen based on the purified protein was developed. |
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| Keywords: | FMDV type C Recombiant P1 Expression biology acting |
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