| 不同浓度CO2对牛卵母细胞体外成熟和受精的影响 |
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| 引用本文： | 朱淑文,华修国,李华威,今野博章,李培昌. 不同浓度CO2对牛卵母细胞体外成熟和受精的影响[J]. 猪业科学, 2003, 20(9): 43-44 |
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| 作者姓名： | 朱淑文 华修国 李华威 今野博章 李培昌 |
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| 作者单位： | 1. 上海交通大学农学院,上海,201101
2. 日本国宇都宫大学农学部,日本,宇都宫,321-8505 |
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| 基金项目： | 上海市农委科技兴农重点攻关项目(BM150162) |
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| 摘 要： | 对牛卵巢卵泡内卵母细胞在不同浓度的CO2培养箱内体外成熟、体外受精后的卵裂率及胚胎体外发育进行了研究。结果显示,含5%小牛血清(CS)的TCM-199内的卵母细胞在2%CO2条件下培养20h后,达到第二次减数分裂期(MⅡ期)的卵子数明显高于在5%CO2条件下培养的卵母细胞数(P<0.05)。两种浓度的CO2条件下培养的牛卵母细胞体外受精后的卵裂率无明显差异,2%CO2浓度下培养的卵母细胞的囊胚发育率高于5%CO2浓度下培养的卵母细胞。
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| 关 键 词： | 牛卵母细胞 第二次减数分裂期 体外受精 卵裂率 囊胚 |
| 文章编号： | 1008-9381(2003)09-0043-02 |
| 修稿时间： | 2003-08-08 |
Effect of Different Concentration of CO2 on the Bovine Oocytes In Vitro Maturation and Fertilization |
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| Zhu Shu-wen Hua Xiu-guo Li Hua-wei Kono Hatsuda Li pei-chang. Effect of Different Concentration of CO2 on the Bovine Oocytes In Vitro Maturation and Fertilization[J]. Swine Industry Science, 2003, 20(9): 43-44 |
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| Authors: | Zhu Shu-wen Hua Xiu-guo Li Hua-wei Kono Hatsuda Li pei-chang |
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| Affiliation: | Zhu Shu-wen 1 Hua Xiu-guo 1 Li Hua-wei 1 Kono Hatsuda 2 Li pei-chang 2 |
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| Abstract: | The purpose of the present experiment was to determine the bovine oocytes in vitro maturation and fertilization in different concentration of CO 2 .The frequency of oocytes at meiosis stage was significantly higher in the2%CO 2 than that in the5%CO 2 (P<0.05).The cleavage rates was no significant difference in the various concentration of CO 2 .But the percentages of developed to blastocyst stage in the2%CO 2 than that in the5%CO 2 . |
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| Keywords: | Bovine oocyte meiosis stage in vitro fertilization cleavage rates blastocyst |
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