Physiological Concentrations of Acute-Phase Proteins and Immunoglobulins in Equine Synovial Fluid |
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| Authors: | Roberta C Basile Guilherme C Ferraz Marsel P Carvalho Raquel M Albernaz Renatha A Araújo José J Fagliari Antonio Queiroz-Neto |
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| Institution: | 1. Faculdade de Ciências Agrárias e Veterinárias, Departamento de Morfologia e Fisiologia Animal, Laboratório de Farmacologia e Fisiologia do Exercício Equino – LAFEQ, UNESP – Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, São Paulo, Brazil;2. Faculdade de Ciências Agrárias e Veterinárias, Departamento de Clínica e Cirurgia Veterinária, UNESP – Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, São Paulo, Brazil |
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| Abstract: | Synovial fluid (SF) is capable of reflecting infectious, immunological, or inflammatory joint conditions in horses by altering its composition and appearance. Although plasma and SF compositions are quantitatively different, this latter compartment reflects changes in plasma macromolecules. Therefore, changes in serum immunoglobulin protein concentrations tend also to alter intracapsular levels. Therefore, it is necessary to know the physiological concentrations of proteins present in SF. The aim of this study was to determine the levels of total protein, albumin, transferrin, haptoglobin, α1-acid glycoprotein, ceruloplasmin, and immunoglobulins A and G in SF of six healthy horses. The synovial proteinogram was obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The SF proteins reached a maximum of 25% of serum concentrations, varying inversely with molecular weight of the protein, except for the ceruloplasmin. |
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| Keywords: | Horse Joint Proteinogram Electrophoresis SDS-polyacrylamide gel electrophoresis |
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