Increased Inflammatory Mediators in Horses Naturally Infected with Trypanosoma vivax. A Preliminary Study |
| |
| Authors: | Aleksandro S. Da Silva Marta M.M.F. Duarte Guilherme V. Bochi Rafael N. Moresco Lenita Moura Stefani Silvia G. Monteiro |
| |
| Affiliation: | 1. Department of Animal Science, University of Santa Catarina State, Chapecó, Santa Catarina, Brazil;2. Lutheran University of Brazil, Santa Maria, Rio Grande do Sul, Brazil;3. Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil;4. Department of Microbiology and Parasitology, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil |
| |
| Abstract: | The aim of this study was to measure the levels of inflammatory mediators in serum from horses naturally infected with Trypanosoma vivax. Banked serum samples collected during a previously reported T. vivax natural infection were used to analyze proinflammatory cytokines such as interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1), interleukin 6 (IL-6), and nitrite/nitrate (NOx) levels. We evaluated 12 serum samples from horses from a farm in southern Brazil, four of which had parasitological and molecular diagnoses for T. vivax and presented with clinical signs of disease. Cytokines were assessed by quantitative sandwich enzyme-linked immunosorbent assay, and NOx was measured using the modified Griess method. Levels of IFN-γ, TNF-α, IL-1, IL-6, and NOx were increased in serum of infected animals compared to that in noninfected animals. Therefore, infection with T. vivax caused an increase in proinflammatory cytokines and nitric oxide content. |
| |
| Keywords: | Horses Trypanosoma vivax IFN-γ TNF-α IL-1 IL-6 NO&bull |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
