灰枣多糖大孔径树脂提纯工艺优化

新郑灰枣富含各种营养成分,特别是多糖具有抗氧化抗癌等许多生物效应。为研究新郑灰枣多糖的提纯工艺,该文在超声波酶法联合提取、乙醇沉淀、超滤膜过滤得到的粗多糖的基础上,筛选出的AB-8大孔树脂纯化多糖,并对树脂的动态吸附解吸特性进行研究。响应曲面法优化AB-8大孔树脂动态吸附工艺条件,最佳动态吸附条件为:上样速率1.5mL/min,料液浓度2.2mg/mL,pH值5.6,最大动态吸附量为19.52mg/g;正交试验优化AB-8大孔树脂动态解吸的最佳工艺条件为:氯化钠浓度为0.4mol/L,乙醇添加量60%,盐酸浓度0.2mol/L,流速1.5mL/min,最优动态解吸率为85.21%。通过树脂纯化,多糖纯度可达88.87%。该红枣多糖提纯技术是一种非常有效的方法,在医疗及保健行业具有巨大的应用潜力和市场。

Optimization of resin based technology for purification of polysaccharides from Ziziphus Jujuba cv. Huizao

Ziziphus Jujuba cv. Xinzheng Huizao is rich in kinds of nutrients, especially polysaccharides, which possess many biological effects on human bodies. However, the purification of polysaccharides from jujube after a primarily extraction step is still a difficult technology. In present research, enzyme combined with ultrasonic wave extraction, ethanol precipitation and ultrafiltration membrane filtration were employed to extract and preliminarily purify the polysaccharides from Ziziphus Jujuba cv. Xinzheng Huizao. The obtained raw polysaccharides were employed for further purification. Through the investigation of static adsorption and desorption of resins, the most suitable macroporous resin for the purification of jujube polysaccharides was selected and its dynamic adsorption and desorption behavior was studied. Furthermore, response surface experiments were conducted to investigate the effects of multifactor on the adsorption properties of macroporous resin for purification of jujube polysaccharide. The optimum adsorption conditions were 1.5 mL/min of in-flux rate, 2.2mg/mL of concentration of the feed liquid, 5.6 of pH value. Under this condition, the maximum dynamic adsorption rate of 19.52 mg/g could be obtained in theory. The optimized technical condition for dynamic desorption was obtained with 0.4 mol/L of NaCl, 60% of ethanol, 0.2 mol/L of HCl and 1.5 mL/min of flow rate. Under this condition, maximum dynamic desorption rate of 85.21% was achieved, and the purity of yield polysaccharides was 88.87%. Taken together, the present optimized technology is a highly effective method for the extraction and purification of polysaccharides from jujube, and has a great application and market potential in the medical and health care industries.