泰安及周边地区禽大肠杆菌分离鉴定及耐药分析

对泰安及周边地区鸡大肠杆菌病的流行情况和致病性大肠杆菌的耐药性状况进行了比较系统的调查分析：对25株鸡源性大肠杆菌进行了分离纯化、生化试验,同时对所分离的菌株进行了耐药性测定及β内酰胺药物耐药基因TEM的检测等研究。结果表明：大肠杆菌对多种抗生素均产生了耐药性,对β－内酰胺类药物的耐药性也相当严重,对在药敏试验中检测出对头孢菌素类药物不敏感的菌株进行TEM基因检测,发现这些菌株均含有耐β-内酰胺酶耐药基因,与Genebank上登录的其他耐β-内酰胺酶耐药基因序列的同源性达到97％以上,仅存在个别的碱基突变。通过研究以期探明地区鸡源性大肠杆菌病的流行情况和细菌的耐药现状．从而为有效控制鸡大肠杆菌病的发生流行提供可靠的理论依据,以便制订出较为科学合理的预防和治疗措施,以提高养殖效益。

Separation,Identification and Drug Resistance Analysis of Avian Pathogenic E.coli in Taian and Surrounding Areas

The study of prevalence of chicken escherichia coli disease and the drug resistance state of pathogenic e.coli in Taian and surrounding areas has been carried on the systematic investigation and analysis. We report the isolation, purification, biochemical test of 25 strains of chicken e.coli,at the same time we has researched on its drug resistance and detection of TEM which is the beta lactam drug resistant gene. Results showed that escherichia coli are insensitive to several antibiotics, especially beta lactam drug. In the drug sensitive test these strains insensitive to cephalosporin drugs contain beta lactamase resistant gene. Compared to beta lactamase resistant gene of Genebank their sequence homology are more than 97% and there is only individual base mutations. Through research to find out the prevalence of chicken escherichia coli disease and the present situation of bacterial drug resistance, even to provide reliable theoretical basis for controlling the chicken escherichia coli disease epidemics effectively. Eventually working out more scientific and reasonable prevention and treatment measures to improve the breeding benefit .