应用RT—PCR方法检测鸭病毒性肝炎新型和I型病毒

鸭病毒性肝炎病毒是危害养鸭业的重要病毒性传染病，主要感染低日龄雏鸭，临床上分离到的病原主要为鸭病毒性肝炎I型病毒和鸭病毒性肝炎新型病毒，为了加快本病的检测速度，尽快确诊病原，根据GeneBank上两种毒株的基因组序列，选择两种毒株的差异基因序列分别设计了一对特异性的检测引物，将两对引物放在一个PCR反应体系中，两种毒株分别进行特异性扩增，根据扩增片段的大小进行鉴别，建立了可以同时检测鸭病毒性肝炎病毒I型和新型毒株的复合RT—PCR方法，该检测方法快速，特异性强，灵敏性高，可以用于鸭病毒性肝炎病毒的分型鉴别检测。

Detection of Duck Hepatitis Virus of New Type and Type I Using RT-PCR

Duck hepatitis virus is an infectious viral disease which jeopardizes the duck industry and mainly affects the young ducklings. The pathogen isolated clinically was mainly duck hepatitis virus type I and new type virus. In order to speed up the disease detection and diagnose the pathogens as soon as possi- ble, according to the genomic sequence of two strains in GeneBank, a pair of specific primers was designed respectively according to the differences between the two sequences, two pairs of primers were used in a PCR reaction system, and two strains were amplified specifically. Identified according to the amplification fragment size, a complex PCR detection method which can simultaneously detect duck hepatitis virus type I and new type strains was established, the detection method is rapid and has strong specificity and high sensitivity, and can be used for identifying the types of duck hepatitis virus detection.