| 木豆叶总黄酮超声提取工艺优化研究 |
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| 引用本文： | 郑菲艳,李爱萍,徐晓俞,赖友辉,郑开斌. 木豆叶总黄酮超声提取工艺优化研究[J]. 福建农业学报, 2013, 0(11): 1149-1153 |
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| 作者姓名： | 郑菲艳 李爱萍 徐晓俞 赖友辉 郑开斌 |
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| 作者单位： | [1]福建农林大学食品科学学院,福建福州350002 [2]福建省农业科学院作物研究所,福建福州350003 [3]福建森辉农牧发展有限公司,福建长汀366300 [4]福建省农业科学院甘蔗研究所,福建漳州363000 |
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| 基金项目： | 福建省农业科技重大专项（2012NZ0002）;福建省区域科技重大项目（2012N3015）;福建省财政专项--福建省农业科学院科技创新团队建设项目（CXTD-1-1301）;福建省农业科学院青年基金项目（2012DG-7） |
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| 摘 要： | 研究超声法提取木豆叶总黄酮的提取工艺，通过单因素、L9（34）正交实验，探讨超声时间、乙醇浓度、料液比对木豆叶总黄酮得率的影响。结果表明，木豆叶总黄酮超声提取最佳条件为：超声时间20min，乙醇浓度70％，料液比为1：30，总黄酮得率达到3．65％。该检测方法稳定性高，重现性好，加样回收率为99．01％～102．72％。
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| 关 键 词： | 木豆叶 总黄酮 超声提取 提取工艺 |
Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves |
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| ZHENG Fei-yan,LI Ai-ping,XU Xiao-yu,LAI You-hui,ZHENG Kai-bin. Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves[J]. Fujian Journal of Agricultural Sciences, 2013, 0(11): 1149-1153 |
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| Authors: | ZHENG Fei-yan LI Ai-ping XU Xiao-yu LAI You-hui ZHENG Kai-bin |
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| Affiliation: | 1. Collegeof Food Science, FujianAgriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Corp Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China; 3. Fujian Senhui Farming and Stock Breeding Development Co. , LTD, Changding, Fujian 366300, China; 4. Sugarcane Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China) |
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| Abstract: | Extracting total flavonoids in pigeon pea leaves by ultrasonic method. Analysis the factors which ultrasonic extraction time, the concentration of ethanol and the solid-liquid ratio condition and L9 （34） orthogonal, to find the optimal conditions of extraction of total flovonoids in pigeon pea leaves. From the orthogonal results of ultrasonic extraction, the optimal conditions of extraction of total flavonoids in pigeon pea leaves are as following： 20 min ultrasonic extraction 70~ ethanol, 1 ： 30 solid-liquid ratio. With above extraction condition the yield of total flavonoids is 3.65 %. The average recovery of the detection method is 99.01 ~- 102.72%, the method has excellent stability and repeatability. |
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| Keywords: | pigeon pea leaves total flavonoids ultrasonic extraction extraction process |
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