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Summary of the first round analyses of the Second International Swine CD Workshop |
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| Authors: | A Saalmüller T Pauly B Aasted K T Jensen D H Sachs S Arn W C Davis Y H Park K McCullough A Summerfield M Murtaugh M S Pampusch K D Burger J Laber J Nielsen M D Pescovitz C Stokes K Haverson P Boyd J K Lunney |
| Institution: | a Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany b Department of Veterinary Virology and Immunology, Royal Veterinary University, Frederiksberg C, Denmark c Transplant Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USA d Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, USA e Institut für Viruskrankheiten und Immunprophylaxe, 3147 Mittelhäusern, Switzerland f Department Veterinary Pathobiology College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA g State Veterinary Institute for Virus Research, Lindholm, 4771 Kalvehave, Denmark h Indiana University, Department of Surgery, University Hospital, Indianapolis, IN, USA i Department Veterinary Medicine, University of Bristol, School of Medicine, Langford, Bristol, UK j USDA ARS, Immunology ad Disease Resistance Laboratory, Beltsville, MD, USA |
| Abstract: | The reactivity of 176 monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, together with 19 internal standards, was analyzed by flow cytometry on 16 different cell types as a means of establishing the proper cell subset for later detailed clustering analyses. The exact CD subset reactivity of the 19 internal standard mAb had been characterized in the First International Swine CD Workshop. The flow cytometric analyses resulted in 40 data sets which were then subjected to statistical clustering using the Leukocyte Typing Database IV (LTDB4) software. As result of this work, 22 clusters were defined. After review of these results, panels of mAb from the defined first round clusters were assigned to cell subsets. The respective mAb in those first round clusters were then distributed to subset group researchers for further examination during the second round of the workshop. |
| Keywords: | Monoclonal antibodies Flow cytometry Clustering analysis |
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