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| 重组抗菌肽LfcinB/LfampinB基因的克隆及表达载体的构建 | |
| 引用本文： | 孙金霞,刘艳环,李海涛,李庆超,张广雷,苗利光. 重组抗菌肽LfcinB/LfampinB基因的克隆及表达载体的构建[J]. 特产研究, 2010, 32(2): 16-18,39 |
| 作者姓名： | 孙金霞 刘艳环 李海涛 李庆超 张广雷 苗利光 |
| 作者单位： | 1. 江苏科技大学,江苏镇江,212018;中国农业科学院特产研究所,吉林吉林,132109 2. 中国农业科学院特产研究所,吉林吉林,132109 3. 江苏科技大学,江苏镇江,212018 |
| 基金项目： | 中国农业科学院特产研究所基金 |
| 摘 要： | 根据GeneBank中提供的牛乳铁蛋白基因序列,设计并合成了重组LfcinB/LfampinB基因,并通过PGEM-Teasy载体扩增。酶切回收的LfcinB/LfampinB片段与中间载体pP6连接。pP6上的启动子、信号肽和调控序列与LfcinB/LfampinB共同切下,获得具有上游调控元件的PLfc/Lfa基因片段。将PLfc/Lfa与pME290表达载体连接,并转化绿脓杆菌。结果表明,本试验成功构建了重组pPLfc/Lfa表达载体。 |
| 关 键 词： | 抗菌肽 克隆 表达载体 构建 |
The Cloing and Expressional Vector Construction of Recombinant Antimicrobial peptide LfcinB/LfampinB Gene |
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| SUN Jin-xia,LIU Yan-huan,LI Hai-tao,Li Qing-chao,ZHANG Guang-lei,MIAO Li-guang. The Cloing and Expressional Vector Construction of Recombinant Antimicrobial peptide LfcinB/LfampinB Gene[J]. Special Wild Economic Animal and Plant Research, 2010, 32(2): 16-18,39 | |
| Authors: | SUN Jin-xia LIU Yan-huan LI Hai-tao Li Qing-chao ZHANG Guang-lei MIAO Li-guang |
| Affiliation: | 1.Jiangsu University of Science and Technology,Zhenjiang 212018,China;2.Institute of Special Wild Economic Animals and Plants CAAS,Jilin 132109,China) |
| Abstract: | the Bovine lactoferrin gene deposited in GeneBank,LfcinB/LfampinB recombinant gene was designed and synthesized,and then amplified by PGEM-T easy plasmid.The purified LfcinB/LfampinB gene by restriction enzyme was linked to pP6 plasmid.Together with LfcinB/LfampinB gene,the promoter,signal peptide and regulator gene which were in pP6 plasmid were digested by restriction enzyme,PLfc/Lfa fragment which had upstream regulatory element was obtained.PLfc/Lfa was linked to pME290 expression plasmid and transformed into competent cells of.The results showed that recombinant expression plasmid pPLfc/Lfa was obtained. |
| Keywords: | Antimicrobial peptide Clone Expressional plasmid Construction |
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