|
|||||
|
|
| 瘦蛋白基因的克隆及在大肠杆菌中的表达 | |
| 引用本文： | 张忠芳,高士争,张曦,李士泽,张玉静. 瘦蛋白基因的克隆及在大肠杆菌中的表达[J]. 中国兽医学报, 2004, 24(5): 463-466 |
| 作者姓名： | 张忠芳 高士争 张曦 李士泽 张玉静 |
| 作者单位： | 1. 北华大学,医学院,吉林,吉林,132001 2. 云南农业大学,云南省动物营养与饲料重点实验室,云南,昆明,650201 3. 黑龙江八一农垦大学,黑龙江,大庆,163319 4. 解放军军需大学,军事兽医系,吉林,长春,130062 |
| 基金项目： | 云南省自然科学基金资助项目 (2 0 0 2 C0 0 45 M) |
| 摘 要： | 采用 RT- PCR方法从猪脂肪组织扩增出瘦蛋白基因 ,将其插入 p MD1 8- T克隆载体 ,经序列分析证明 ,所获得的目的基因为 4 4 1 bp,与预期大小一致。提取质粒后用 Eco R 和 Xho 双酶切 ,克隆入 p ET- 2 8a表达载体 ,将阳性重组质粒转化表达受体菌 E.coli BL 2 1 ( DE3) ,经 IPTG诱导 ,SDS- PAGE检测 ,证明在 E.coli BL 2 1中正确表达了重组猪瘦蛋白 |
| 关 键 词： | 肥胖基因 瘦蛋白 原核表达 |
| 文章编号： | 1005-4545(2004)05-0463-04 |
| 修稿时间： | 2003-10-16 |
Cloning of Porcine Leptin Gene and Its Expression in E.coli |
|
| ZHANG Zhong-fang,GAO Shi-zheng. Cloning of Porcine Leptin Gene and Its Expression in E.coli[J]. Chinese Journal of Veterinary Science, 2004, 24(5): 463-466 | |
| Authors: | ZHANG Zhong-fang GAO Shi-zheng |
| Affiliation: | ZHANG Zhong-fang~1,GAO Shi-zheng~ |
| Abstract: | The porcine leptin gene was amplified by RT-PCR and the PCR product was cloned into a clone vector pMD18-T. Sequence analysis showed that the gene obtained was 441 bp long, with 99% homology with porcine leptin gene. Then the gene was subcloned into an expression vector pET-28a in correct reading-frame. The positive recombinant plasmids were then transformed into host strain E.coli BL21(DE3) and induced by IPTG. The recombinant protein was expressed in E.coli successfully as confirmed by SDS-PAGE. |
| Keywords: | obese gene leptin prokaryotic expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |